Pseudomonas aeruginosa hemolytic phospholipase C suppresses neutrophil respiratory burst activity

Lance S. Terada, Kristine A. Johansen, Sogol Nowbar, Adriana I. Vasil, Michael L. Vasil

Research output: Contribution to journalArticle

69 Citations (Scopus)

Abstract

Pseudomonas aeruginosa is a persistent pathogen in the airways of patients with cystic fibrosis or bronchiectasis from other causes and appears to have evolved strategies to survive the inflammatory response of the host. We hypothesized that the secreted hemolytic phospholipase C (PLC) of P. aeruginosa (PlcHR) would decrease neutrophil respiratory burst activity. We found that while intact wild-type P. aeruginosa cells stimulated moderate respiratory burst activity from human neutrophils, an isogenic mutant pseudomonas (ΔHR strain) containing a targeted deletion of the plcHR operon induced a much more robust oxidative burst from neutrophils. In contrast, a second pseudomonas mutant (AN) containing a disruption in the gene encoding the nonhemolytic PLC (PlcN) was not different from the wild type in stimulating neutrophil O2 .- production. Readdition of purified PlcHR to the ΔHR strain suppressed neutrophil O2 .- production to levels stimulated by wild-type bacteria. Interestingly, purified PlcHR decreased phorbol myristate acetate (PMA)- but not formyl methionyl-leucyl-proline (fMLP)- induced respiratory burst activity, suggesting interference by PlcHR with a protein kinase C (PKC)-specific signaling pathway. Accordingly, the PKC inhibitor bisindolylmaleimide inhibited the oxidative burst induced by either PMA or intact pseudomonas, but not by fMLP, whereas the p38 kinase inhibitor SB-203580 fully inhibited the respiratory burst induced by tMLP or the PlcHR- replete wild-type bacteria, but not PMA or the PlcHR-deficient ΔHR bacterial mutant. We conclude that expression of PlcHR by P. aeruginosa suppresses bacterium-induced neutrophil respiratory burst by interfering with a PKC- dependent, non-p38 kinase-dependent pathway.

Original languageEnglish (US)
Pages (from-to)2371-2376
Number of pages6
JournalInfection and Immunity
Volume67
Issue number5
StatePublished - May 1999

Fingerprint

Respiratory Burst
Type C Phospholipases
Pseudomonas aeruginosa
Neutrophils
Tetradecanoylphorbol Acetate
Pseudomonas
Protein Kinase C
Bacteria
Proline
Phosphotransferases
Bronchiectasis
Protein C Inhibitor
Operon
Protein Kinase Inhibitors
Human Activities
Cystic Fibrosis
Genes

ASJC Scopus subject areas

  • Immunology

Cite this

Terada, L. S., Johansen, K. A., Nowbar, S., Vasil, A. I., & Vasil, M. L. (1999). Pseudomonas aeruginosa hemolytic phospholipase C suppresses neutrophil respiratory burst activity. Infection and Immunity, 67(5), 2371-2376.

Pseudomonas aeruginosa hemolytic phospholipase C suppresses neutrophil respiratory burst activity. / Terada, Lance S.; Johansen, Kristine A.; Nowbar, Sogol; Vasil, Adriana I.; Vasil, Michael L.

In: Infection and Immunity, Vol. 67, No. 5, 05.1999, p. 2371-2376.

Research output: Contribution to journalArticle

Terada, LS, Johansen, KA, Nowbar, S, Vasil, AI & Vasil, ML 1999, 'Pseudomonas aeruginosa hemolytic phospholipase C suppresses neutrophil respiratory burst activity', Infection and Immunity, vol. 67, no. 5, pp. 2371-2376.
Terada, Lance S. ; Johansen, Kristine A. ; Nowbar, Sogol ; Vasil, Adriana I. ; Vasil, Michael L. / Pseudomonas aeruginosa hemolytic phospholipase C suppresses neutrophil respiratory burst activity. In: Infection and Immunity. 1999 ; Vol. 67, No. 5. pp. 2371-2376.
@article{e1f3781c152f45bf9ab148dddedc50aa,
title = "Pseudomonas aeruginosa hemolytic phospholipase C suppresses neutrophil respiratory burst activity",
abstract = "Pseudomonas aeruginosa is a persistent pathogen in the airways of patients with cystic fibrosis or bronchiectasis from other causes and appears to have evolved strategies to survive the inflammatory response of the host. We hypothesized that the secreted hemolytic phospholipase C (PLC) of P. aeruginosa (PlcHR) would decrease neutrophil respiratory burst activity. We found that while intact wild-type P. aeruginosa cells stimulated moderate respiratory burst activity from human neutrophils, an isogenic mutant pseudomonas (ΔHR strain) containing a targeted deletion of the plcHR operon induced a much more robust oxidative burst from neutrophils. In contrast, a second pseudomonas mutant (AN) containing a disruption in the gene encoding the nonhemolytic PLC (PlcN) was not different from the wild type in stimulating neutrophil O2 .- production. Readdition of purified PlcHR to the ΔHR strain suppressed neutrophil O2 .- production to levels stimulated by wild-type bacteria. Interestingly, purified PlcHR decreased phorbol myristate acetate (PMA)- but not formyl methionyl-leucyl-proline (fMLP)- induced respiratory burst activity, suggesting interference by PlcHR with a protein kinase C (PKC)-specific signaling pathway. Accordingly, the PKC inhibitor bisindolylmaleimide inhibited the oxidative burst induced by either PMA or intact pseudomonas, but not by fMLP, whereas the p38 kinase inhibitor SB-203580 fully inhibited the respiratory burst induced by tMLP or the PlcHR- replete wild-type bacteria, but not PMA or the PlcHR-deficient ΔHR bacterial mutant. We conclude that expression of PlcHR by P. aeruginosa suppresses bacterium-induced neutrophil respiratory burst by interfering with a PKC- dependent, non-p38 kinase-dependent pathway.",
author = "Terada, {Lance S.} and Johansen, {Kristine A.} and Sogol Nowbar and Vasil, {Adriana I.} and Vasil, {Michael L.}",
year = "1999",
month = "5",
language = "English (US)",
volume = "67",
pages = "2371--2376",
journal = "Infection and Immunity",
issn = "0019-9567",
publisher = "American Society for Microbiology",
number = "5",

}

TY - JOUR

T1 - Pseudomonas aeruginosa hemolytic phospholipase C suppresses neutrophil respiratory burst activity

AU - Terada, Lance S.

AU - Johansen, Kristine A.

AU - Nowbar, Sogol

AU - Vasil, Adriana I.

AU - Vasil, Michael L.

PY - 1999/5

Y1 - 1999/5

N2 - Pseudomonas aeruginosa is a persistent pathogen in the airways of patients with cystic fibrosis or bronchiectasis from other causes and appears to have evolved strategies to survive the inflammatory response of the host. We hypothesized that the secreted hemolytic phospholipase C (PLC) of P. aeruginosa (PlcHR) would decrease neutrophil respiratory burst activity. We found that while intact wild-type P. aeruginosa cells stimulated moderate respiratory burst activity from human neutrophils, an isogenic mutant pseudomonas (ΔHR strain) containing a targeted deletion of the plcHR operon induced a much more robust oxidative burst from neutrophils. In contrast, a second pseudomonas mutant (AN) containing a disruption in the gene encoding the nonhemolytic PLC (PlcN) was not different from the wild type in stimulating neutrophil O2 .- production. Readdition of purified PlcHR to the ΔHR strain suppressed neutrophil O2 .- production to levels stimulated by wild-type bacteria. Interestingly, purified PlcHR decreased phorbol myristate acetate (PMA)- but not formyl methionyl-leucyl-proline (fMLP)- induced respiratory burst activity, suggesting interference by PlcHR with a protein kinase C (PKC)-specific signaling pathway. Accordingly, the PKC inhibitor bisindolylmaleimide inhibited the oxidative burst induced by either PMA or intact pseudomonas, but not by fMLP, whereas the p38 kinase inhibitor SB-203580 fully inhibited the respiratory burst induced by tMLP or the PlcHR- replete wild-type bacteria, but not PMA or the PlcHR-deficient ΔHR bacterial mutant. We conclude that expression of PlcHR by P. aeruginosa suppresses bacterium-induced neutrophil respiratory burst by interfering with a PKC- dependent, non-p38 kinase-dependent pathway.

AB - Pseudomonas aeruginosa is a persistent pathogen in the airways of patients with cystic fibrosis or bronchiectasis from other causes and appears to have evolved strategies to survive the inflammatory response of the host. We hypothesized that the secreted hemolytic phospholipase C (PLC) of P. aeruginosa (PlcHR) would decrease neutrophil respiratory burst activity. We found that while intact wild-type P. aeruginosa cells stimulated moderate respiratory burst activity from human neutrophils, an isogenic mutant pseudomonas (ΔHR strain) containing a targeted deletion of the plcHR operon induced a much more robust oxidative burst from neutrophils. In contrast, a second pseudomonas mutant (AN) containing a disruption in the gene encoding the nonhemolytic PLC (PlcN) was not different from the wild type in stimulating neutrophil O2 .- production. Readdition of purified PlcHR to the ΔHR strain suppressed neutrophil O2 .- production to levels stimulated by wild-type bacteria. Interestingly, purified PlcHR decreased phorbol myristate acetate (PMA)- but not formyl methionyl-leucyl-proline (fMLP)- induced respiratory burst activity, suggesting interference by PlcHR with a protein kinase C (PKC)-specific signaling pathway. Accordingly, the PKC inhibitor bisindolylmaleimide inhibited the oxidative burst induced by either PMA or intact pseudomonas, but not by fMLP, whereas the p38 kinase inhibitor SB-203580 fully inhibited the respiratory burst induced by tMLP or the PlcHR- replete wild-type bacteria, but not PMA or the PlcHR-deficient ΔHR bacterial mutant. We conclude that expression of PlcHR by P. aeruginosa suppresses bacterium-induced neutrophil respiratory burst by interfering with a PKC- dependent, non-p38 kinase-dependent pathway.

UR - http://www.scopus.com/inward/record.url?scp=0032947599&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032947599&partnerID=8YFLogxK

M3 - Article

C2 - 10225897

AN - SCOPUS:0032947599

VL - 67

SP - 2371

EP - 2376

JO - Infection and Immunity

JF - Infection and Immunity

SN - 0019-9567

IS - 5

ER -