PspA, a surface protein of Streptococcus pneumonia, is capable of eliciting protection against pneumococci of more than one capsular type

L. S. McDaniel, J. S. Sheffield, P. Delucchi, D. E. Briles

Research output: Contribution to journalArticle

152 Citations (Scopus)

Abstract

Monoclonal antibodies against pneumococcal surface protein A (PspA) have been shown to protect mice from fatal pneumococcal infection. PspA is highly variable serologically, raising the possibility that pA from one strain might not be able to elicit protective responses against strains which possess serologically different PspA. We have prepared a λgt11 library of pneumococcal genomic DNA and identified a clone expressing PspA. The recombinant PspA in this phage lysate elicited protection against pneumococcal infections with three strains of two different capsular serotypes. This finding demonstrated that PspA could elicit a protective response in the absence of other pneumococcal antigens. The observed protection was probably antibody mediated because it could be passively transferred with immune sera. Lambda lysates producing pneumococcal proteins other than PspA failed to elicit protection against fatal pneumococcal infection.

Original languageEnglish (US)
Pages (from-to)222-228
Number of pages7
JournalInfection and Immunity
Volume59
Issue number1
StatePublished - 1991

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Streptococcus pneumoniae
Membrane Proteins
Pneumococcal Infections
Genomic Library
pneumococcal surface protein A
Bacteriophages
Immune Sera
Clone Cells
Monoclonal Antibodies
Antigens
Antibodies
DNA
Proteins

ASJC Scopus subject areas

  • Immunology

Cite this

PspA, a surface protein of Streptococcus pneumonia, is capable of eliciting protection against pneumococci of more than one capsular type. / McDaniel, L. S.; Sheffield, J. S.; Delucchi, P.; Briles, D. E.

In: Infection and Immunity, Vol. 59, No. 1, 1991, p. 222-228.

Research output: Contribution to journalArticle

McDaniel, L. S. ; Sheffield, J. S. ; Delucchi, P. ; Briles, D. E. / PspA, a surface protein of Streptococcus pneumonia, is capable of eliciting protection against pneumococci of more than one capsular type. In: Infection and Immunity. 1991 ; Vol. 59, No. 1. pp. 222-228.
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