Purification and adoptive transfer of group 3 gut innate lymphoid cells

Xiaohuan Guo; Kevin Muite; Joanna Wroblewska; Yang Xin Fu

doi:10.1007/978-1-4939-3603-8_18

Purification and adoptive transfer of group 3 gut innate lymphoid cells

Xiaohuan Guo, Kevin Muite, Joanna Wroblewska, Yang Xin Fu

Research output: Contribution to journal › Article › peer-review

12 Scopus citations

Abstract

Recent studies have identified several related but distinct innate lymphoid cells (ILCs) populations that are relatively enriched in the intestinal mucosal and protect the host from various infections. Among ILCs, group 3 ILCs (ILC3s) produce lymphotoxin and IL-22, and play important roles in the development of the immune system and host-bacteria interactions. Here, we describe methods for the isolation and purification of ILC3s from the mouse intestine, and the adoptive transfer of purified ILC3s into recipient mice.

Original language	English (US)
Pages (from-to)	189-196
Number of pages	8
Journal	Methods in Molecular Biology
Volume	1422
DOIs	https://doi.org/10.1007/978-1-4939-3603-8_18
State	Published - 2016

Keywords

Adoptive transfer
Group 3 Innate lymphoid cells
Intestine
Purification
Rag1

ASJC Scopus subject areas

Molecular Biology
Genetics

Access to Document

10.1007/978-1-4939-3603-8_18

Cite this

@article{a73d9d27624243b6aefb2b72b3019175,

title = "Purification and adoptive transfer of group 3 gut innate lymphoid cells",

abstract = "Recent studies have identified several related but distinct innate lymphoid cells (ILCs) populations that are relatively enriched in the intestinal mucosal and protect the host from various infections. Among ILCs, group 3 ILCs (ILC3s) produce lymphotoxin and IL-22, and play important roles in the development of the immune system and host-bacteria interactions. Here, we describe methods for the isolation and purification of ILC3s from the mouse intestine, and the adoptive transfer of purified ILC3s into recipient mice.",

keywords = "Adoptive transfer, Group 3 Innate lymphoid cells, Intestine, Purification, Rag1",

author = "Xiaohuan Guo and Kevin Muite and Joanna Wroblewska and Fu, {Yang Xin}",

note = "Funding Information: This work was supported by the National Natural Science Foundation of ChinaNSFC (31570923 to X.G.) and National Institutes of HealthNIH grant (DK100427-01A1 to Y.X.F.). Publisher Copyright: {\textcopyright} Springer Science+Business Media New York 2016.",

year = "2016",

doi = "10.1007/978-1-4939-3603-8_18",

language = "English (US)",

volume = "1422",

pages = "189--196",

journal = "Methods in Molecular Biology",

issn = "1064-3745",

publisher = "Humana Press",

}

TY - JOUR

T1 - Purification and adoptive transfer of group 3 gut innate lymphoid cells

AU - Guo, Xiaohuan

AU - Muite, Kevin

AU - Wroblewska, Joanna

AU - Fu, Yang Xin

N1 - Funding Information: This work was supported by the National Natural Science Foundation of ChinaNSFC (31570923 to X.G.) and National Institutes of HealthNIH grant (DK100427-01A1 to Y.X.F.). Publisher Copyright: © Springer Science+Business Media New York 2016.

PY - 2016

Y1 - 2016

N2 - Recent studies have identified several related but distinct innate lymphoid cells (ILCs) populations that are relatively enriched in the intestinal mucosal and protect the host from various infections. Among ILCs, group 3 ILCs (ILC3s) produce lymphotoxin and IL-22, and play important roles in the development of the immune system and host-bacteria interactions. Here, we describe methods for the isolation and purification of ILC3s from the mouse intestine, and the adoptive transfer of purified ILC3s into recipient mice.

AB - Recent studies have identified several related but distinct innate lymphoid cells (ILCs) populations that are relatively enriched in the intestinal mucosal and protect the host from various infections. Among ILCs, group 3 ILCs (ILC3s) produce lymphotoxin and IL-22, and play important roles in the development of the immune system and host-bacteria interactions. Here, we describe methods for the isolation and purification of ILC3s from the mouse intestine, and the adoptive transfer of purified ILC3s into recipient mice.

KW - Adoptive transfer

KW - Group 3 Innate lymphoid cells

KW - Intestine

KW - Purification

KW - Rag1

UR - http://www.scopus.com/inward/record.url?scp=84990050113&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84990050113&partnerID=8YFLogxK

U2 - 10.1007/978-1-4939-3603-8_18

DO - 10.1007/978-1-4939-3603-8_18

M3 - Article

C2 - 27246034

AN - SCOPUS:84990050113

SN - 1064-3745

VL - 1422

SP - 189

EP - 196

JO - Methods in Molecular Biology

JF - Methods in Molecular Biology

ER -

Purification and adoptive transfer of group 3 gut innate lymphoid cells

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this