Purification and adoptive transfer of group 3 gut innate lymphoid cells

Xiaohuan Guo, Kevin Muite, Joanna Wroblewska, Yang Xin Fu

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

Recent studies have identified several related but distinct innate lymphoid cells (ILCs) populations that are relatively enriched in the intestinal mucosal and protect the host from various infections. Among ILCs, group 3 ILCs (ILC3s) produce lymphotoxin and IL-22, and play important roles in the development of the immune system and host-bacteria interactions. Here, we describe methods for the isolation and purification of ILC3s from the mouse intestine, and the adoptive transfer of purified ILC3s into recipient mice.

Original languageEnglish (US)
Pages (from-to)189-196
Number of pages8
JournalMethods in Molecular Biology
Volume1422
DOIs
StatePublished - Jan 1 2016

Keywords

  • Adoptive transfer
  • Group 3 Innate lymphoid cells
  • Intestine
  • Purification
  • Rag1

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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