Purification and assay of the human INO80 and SRCAP chromatin remodeling complexes

Yong Cai, Jingji Jin, Aaron J. Gottschalk, Tingting Yao, Joan W. Conaway, Ronald C. Conaway

Research output: Contribution to journalArticlepeer-review

27 Scopus citations

Abstract

Chromatin modifying and remodeling enzymes play critical roles in many aspects of chromosome biology including transcription, replication, recombination, and repair. Our laboratory recently identified and characterized two multisubunit human chromatin remodeling enzymes designated the INO80 and SRCAP complexes. Mechanistic studies revealed that the human INO80 complex catalyzes nucleosome sliding and the SRCAP complex catalyzes ATP-dependent exchange of histone H2A/H2B dimers containing the histone variant H2A.Z into nucleosomes. Here we describe methods for purification and assay of the INO80 and SRCAP chromatin remodeling complexes.

Original languageEnglish (US)
Pages (from-to)312-317
Number of pages6
JournalMethods
Volume40
Issue number4
DOIs
StatePublished - Dec 2006
Externally publishedYes

Keywords

  • Chromatin
  • Chromatin remodeler
  • Histone
  • Histone exchange
  • Nucleosome

ASJC Scopus subject areas

  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)

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