Purification and cDNA cloning of an insecticidal protein from the venom of the scorpion Orthochirus scrobiculosus

Sergey Kozlov; Alexey Lipkin; Elena Nosyreva; Andrew Blake; John D. Windass; Eugene Grishin

doi:10.1016/S0041-0101(99)00164-6

Purification and cDNA cloning of an insecticidal protein from the venom of the scorpion Orthochirus scrobiculosus

Sergey Kozlov, Alexey Lipkin, Elena Nosyreva, Andrew Blake, John D. Windass, Eugene Grishin

Neuroscience

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

Injection of crude venom from the scorpion Orthochirus scrobiculosus into larvae of Heliothis virescens (Lepidoptera: Noctuidae) caused trembling and uncoordinated movement before development of a progressive and prolonged flaccid paralysis. The isolation of the toxin (OsI-1) responsible for this effect of O. scrobiculosus venom is described. The molecular mass of OsI-1 toxin was 6994 Da, as determined by desorption mass spectroscopy. The complete primary structure of OsI-1 was deduced from the sequence of cDNA clones obtained by rapid amplification of cDNA ends (RACE) PCR. Comparison of the deduced amino acid sequence of OsI-1 with those of other insecticidal scorpion toxins indicates that it is a sodium (Na⁺) channel active depressant insect-selective toxin. The analysis of amino acid sequence of the toxin in conjunction with mass spectroscopy data indicates post-translational modification in maturation with the removal of 3 C-terminal amino acids and amidation of the C-terminus. Copyright (C) 1999 Elsevier Science Ltd.

Original language	English (US)
Pages (from-to)	361-371
Number of pages	11
Journal	Toxicon
Volume	38
Issue number	3
DOIs	https://doi.org/10.1016/S0041-0101(99)00164-6
State	Published - Mar 2000

ASJC Scopus subject areas

Toxicology

Access to Document

10.1016/S0041-0101(99)00164-6

Cite this

@article{51ee760fa08e4841a8fa50d1cd55ea2b,

title = "Purification and cDNA cloning of an insecticidal protein from the venom of the scorpion Orthochirus scrobiculosus",

abstract = "Injection of crude venom from the scorpion Orthochirus scrobiculosus into larvae of Heliothis virescens (Lepidoptera: Noctuidae) caused trembling and uncoordinated movement before development of a progressive and prolonged flaccid paralysis. The isolation of the toxin (OsI-1) responsible for this effect of O. scrobiculosus venom is described. The molecular mass of OsI-1 toxin was 6994 Da, as determined by desorption mass spectroscopy. The complete primary structure of OsI-1 was deduced from the sequence of cDNA clones obtained by rapid amplification of cDNA ends (RACE) PCR. Comparison of the deduced amino acid sequence of OsI-1 with those of other insecticidal scorpion toxins indicates that it is a sodium (Na+) channel active depressant insect-selective toxin. The analysis of amino acid sequence of the toxin in conjunction with mass spectroscopy data indicates post-translational modification in maturation with the removal of 3 C-terminal amino acids and amidation of the C-terminus. Copyright (C) 1999 Elsevier Science Ltd.",

author = "Sergey Kozlov and Alexey Lipkin and Elena Nosyreva and Andrew Blake and Windass, {John D.} and Eugene Grishin",

note = "Funding Information: This work was supported by Zeneca Agrochemicals UK.",

year = "2000",

month = mar,

doi = "10.1016/S0041-0101(99)00164-6",

language = "English (US)",

volume = "38",

pages = "361--371",

journal = "Toxicon",

issn = "0041-0101",

publisher = "Elsevier Limited",

number = "3",

}

TY - JOUR

T1 - Purification and cDNA cloning of an insecticidal protein from the venom of the scorpion Orthochirus scrobiculosus

AU - Kozlov, Sergey

AU - Lipkin, Alexey

AU - Nosyreva, Elena

AU - Blake, Andrew

AU - Windass, John D.

AU - Grishin, Eugene

N1 - Funding Information: This work was supported by Zeneca Agrochemicals UK.

PY - 2000/3

Y1 - 2000/3

N2 - Injection of crude venom from the scorpion Orthochirus scrobiculosus into larvae of Heliothis virescens (Lepidoptera: Noctuidae) caused trembling and uncoordinated movement before development of a progressive and prolonged flaccid paralysis. The isolation of the toxin (OsI-1) responsible for this effect of O. scrobiculosus venom is described. The molecular mass of OsI-1 toxin was 6994 Da, as determined by desorption mass spectroscopy. The complete primary structure of OsI-1 was deduced from the sequence of cDNA clones obtained by rapid amplification of cDNA ends (RACE) PCR. Comparison of the deduced amino acid sequence of OsI-1 with those of other insecticidal scorpion toxins indicates that it is a sodium (Na+) channel active depressant insect-selective toxin. The analysis of amino acid sequence of the toxin in conjunction with mass spectroscopy data indicates post-translational modification in maturation with the removal of 3 C-terminal amino acids and amidation of the C-terminus. Copyright (C) 1999 Elsevier Science Ltd.

AB - Injection of crude venom from the scorpion Orthochirus scrobiculosus into larvae of Heliothis virescens (Lepidoptera: Noctuidae) caused trembling and uncoordinated movement before development of a progressive and prolonged flaccid paralysis. The isolation of the toxin (OsI-1) responsible for this effect of O. scrobiculosus venom is described. The molecular mass of OsI-1 toxin was 6994 Da, as determined by desorption mass spectroscopy. The complete primary structure of OsI-1 was deduced from the sequence of cDNA clones obtained by rapid amplification of cDNA ends (RACE) PCR. Comparison of the deduced amino acid sequence of OsI-1 with those of other insecticidal scorpion toxins indicates that it is a sodium (Na+) channel active depressant insect-selective toxin. The analysis of amino acid sequence of the toxin in conjunction with mass spectroscopy data indicates post-translational modification in maturation with the removal of 3 C-terminal amino acids and amidation of the C-terminus. Copyright (C) 1999 Elsevier Science Ltd.

UR - http://www.scopus.com/inward/record.url?scp=0033990624&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033990624&partnerID=8YFLogxK

U2 - 10.1016/S0041-0101(99)00164-6

DO - 10.1016/S0041-0101(99)00164-6

M3 - Article

C2 - 10669025

AN - SCOPUS:0033990624

SN - 0041-0101

VL - 38

SP - 361

EP - 371

JO - Toxicon

JF - Toxicon

IS - 3

ER -

Purification and cDNA cloning of an insecticidal protein from the venom of the scorpion Orthochirus scrobiculosus

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this