Purification and characterization of a calcium-dependent protease from rat liver

George N. DeMartino, Dorothy E. Croall

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Abstract

A calcium-dependent protease, previously identified in rat liver and designated peak II [DeMartino, G. N. (1981) Arch. Biochem. Biophys. 211, 253-257], was purified and characterized. The calcium-dependent proteolytic activity was accounted for by an 80 000-dalton protein. Depending on the method of purification, we found that this protease could be associated with a 28 000-dalton subunit, which was devoid of protease activity. The catalytic characteristics of the two different forms of the protease were indistinguishable. Each was half-maximally activated by approximately 250 μM calcium.

Original languageEnglish (US)
Pages (from-to)6287-6291
Number of pages5
JournalBiochemistry
Volume22
Issue number26
StatePublished - 1983

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Liver
Purification
Rats
Peptide Hydrolases
Calcium
Arches
Proteins

ASJC Scopus subject areas

  • Biochemistry

Cite this

Purification and characterization of a calcium-dependent protease from rat liver. / DeMartino, George N.; Croall, Dorothy E.

In: Biochemistry, Vol. 22, No. 26, 1983, p. 6287-6291.

Research output: Contribution to journalArticle

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