Abstract
This chapter focuses on the methods for purifying resting antigen-specific B cells so that their activation by antigens and T cells can be assessed. Many methods for studying the physiology and activation of B lymphocytes are available. The chapter describes a method for purifying a population of TNP-specific B cells (TNP-ABC) from spleens of normal or primed mice. These cells are suitable for the analysis of early activation events of B cells in response to antigen. However, to interpret such data, an accurate measurement of the purity of the enriched population is required. The antigen-binding capacity of these cells as measured by rosetting or binding fluorescent antigen gives a far higher estimate of purity than functional assays, such as proliferation, plaque formation, and an enumeration of clones in the splenic focus assay.
Original language | English (US) |
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Pages (from-to) | 316-325 |
Number of pages | 10 |
Journal | Methods in Enzymology |
Volume | 150 |
Issue number | C |
DOIs | |
State | Published - Jan 1987 |
ASJC Scopus subject areas
- Biochemistry
- Molecular Biology