Purification and characterization of antigen-binding virgin and memory B cells

C. D. Myers, V. M. Sanders, E. Yefenof, K. G. Oliver, J. W. Uhr, E. S. Vitetta

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Abstract

Many methods for studying the physiology and activation of B lymphocytes are currently available. A brief review of the contents of this volume indicates that it is possible to grow B cells from bone marrow precursors, to maintain B cells in culture, to clone B cells which are reactive with mitogens or antigens, and to measure responses to these various stimuli. In the present chapter, we focus on the methods for purifying resting antigen-specific B cells so that their activation by antigens and T cells can be assessed.

Original languageEnglish (US)
Pages (from-to)316-325
Number of pages10
JournalMethods in Enzymology
Volume150
Publication statusPublished - 1987

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ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

Cite this

Myers, C. D., Sanders, V. M., Yefenof, E., Oliver, K. G., Uhr, J. W., & Vitetta, E. S. (1987). Purification and characterization of antigen-binding virgin and memory B cells. Methods in Enzymology, 150, 316-325.