Many methods for studying the physiology and activation of B lymphocytes are currently available. A brief review of the contents of this volume indicates that it is possible to grow B cells from bone marrow precursors, to maintain B cells in culture, to clone B cells which are reactive with mitogens or antigens, and to measure responses to these various stimuli. In the present chapter, we focus on the methods for purifying resting antigen-specific B cells so that their activation by antigens and T cells can be assessed.
|Original language||English (US)|
|Number of pages||10|
|Journal||Methods in Enzymology|
|Publication status||Published - 1987|
ASJC Scopus subject areas
- Molecular Biology