Purification and characterization of antigen-binding virgin and memory B cells

C. D. Myers, V. M. Sanders, E. Yefenof, K. G. Oliver, J. W. Uhr, E. S. Vitetta

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Many methods for studying the physiology and activation of B lymphocytes are currently available. A brief review of the contents of this volume indicates that it is possible to grow B cells from bone marrow precursors, to maintain B cells in culture, to clone B cells which are reactive with mitogens or antigens, and to measure responses to these various stimuli. In the present chapter, we focus on the methods for purifying resting antigen-specific B cells so that their activation by antigens and T cells can be assessed.

Original languageEnglish (US)
Pages (from-to)316-325
Number of pages10
JournalMethods in Enzymology
Volume150
StatePublished - 1987

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Purification
B-Lymphocytes
Cells
Antigens
Data storage equipment
CD27 Antigens
Lymphocytes
Physiology
Mitogens
Cell culture
Bone
Chemical activation
Clone Cells
Cell Culture Techniques
Bone Marrow

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

Cite this

Myers, C. D., Sanders, V. M., Yefenof, E., Oliver, K. G., Uhr, J. W., & Vitetta, E. S. (1987). Purification and characterization of antigen-binding virgin and memory B cells. Methods in Enzymology, 150, 316-325.

Purification and characterization of antigen-binding virgin and memory B cells. / Myers, C. D.; Sanders, V. M.; Yefenof, E.; Oliver, K. G.; Uhr, J. W.; Vitetta, E. S.

In: Methods in Enzymology, Vol. 150, 1987, p. 316-325.

Research output: Contribution to journalArticle

Myers, CD, Sanders, VM, Yefenof, E, Oliver, KG, Uhr, JW & Vitetta, ES 1987, 'Purification and characterization of antigen-binding virgin and memory B cells', Methods in Enzymology, vol. 150, pp. 316-325.
Myers, C. D. ; Sanders, V. M. ; Yefenof, E. ; Oliver, K. G. ; Uhr, J. W. ; Vitetta, E. S. / Purification and characterization of antigen-binding virgin and memory B cells. In: Methods in Enzymology. 1987 ; Vol. 150. pp. 316-325.
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