Purification and characterization of PSP-I and PSP-II, two major proteins from porcine seminal plasma

Kay J. Rutherfurd, Kristine M. Swiderek, Carla B. Green, Shiuan Chen, John E. Shively, Simon C M Kwok

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

Two major glycoproteins, designated PSP-I and PSP-II, were purified from porcine seminal plasma by ammonium sulfate fractionation, CM-cellulose chromatography, gel filtration on Sephadex G-75 (superfine), and reverse phase high performance liquid chromatography. These two proteins exist in several forms differing mainly in the carbohydrate moiety. The complete amino acid sequence of PSP-I has been determined by automated Edman degradation of peptides generated by proteolytic digestion and cyanogen bromide cleavage. The protein is 109 residues long and has a single glycosylation site at the asparagine residue at position 47. In addition, the N-terminal sequence of PSP-II has also been determined. PSP-I is a unique protein; a sequence homology search using the protein data base did not reveal any significant homology with other proteins. PSP-II shares 50% sequence homology with a family of zona pellucida-binding glycoproteins at the N-terminus.

Original languageEnglish (US)
Pages (from-to)352-359
Number of pages8
JournalArchives of Biochemistry and Biophysics
Volume295
Issue number2
DOIs
StatePublished - 1992

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Seminal Plasma Proteins
Purification
Swine
Plasmas
Proteins
Sequence Homology
Glycoproteins
Glycosylation
Cyanogen Bromide
Asparagine
Ammonium Sulfate
High performance liquid chromatography
Fractionation
Chromatography
Cellulose
Reverse-Phase Chromatography
Semen
Gels
Gel Chromatography
Carbohydrates

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Purification and characterization of PSP-I and PSP-II, two major proteins from porcine seminal plasma. / Rutherfurd, Kay J.; Swiderek, Kristine M.; Green, Carla B.; Chen, Shiuan; Shively, John E.; Kwok, Simon C M.

In: Archives of Biochemistry and Biophysics, Vol. 295, No. 2, 1992, p. 352-359.

Research output: Contribution to journalArticle

Rutherfurd, Kay J. ; Swiderek, Kristine M. ; Green, Carla B. ; Chen, Shiuan ; Shively, John E. ; Kwok, Simon C M. / Purification and characterization of PSP-I and PSP-II, two major proteins from porcine seminal plasma. In: Archives of Biochemistry and Biophysics. 1992 ; Vol. 295, No. 2. pp. 352-359.
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