Purification and characterization of recombinant porcine prorelaxin expressed in Escherichia coli

G. Kesava Reddy, Sripad Gunwar, Carla B. Green, David T W Fei, Anthony B. Chen, Simon C M Kwok

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

In this report we describe the purification and characterization of recombinant porcine prorelaxin expressed in Escherichia coli. Nucleotide sequence encoding porcine prorelaxin was inserted into an E. coli expression vector, pOTS, and the recombinant plasmid was transformed into the E. coli host (AR 120). Upon induction with nalidixic acid, the 19-kDa recombinant porcine prorelaxin was produced at a level of approximately 8% of the total accumulated cell protein. The recombinant prorelaxin was purified to homogeneity by CM-cellulose chromatography and reversed-phase HPLC, after refolding in the presence of reduced and oxidized glutathione and a low concentration of guanidine-HCl. The identity of the recombinant prorelaxin was confirmed by the correct size, immunoreactivity with antibodies against native porcine relaxin, and direct amino-terminal sequence analysis. Furthermore, the purified recombinant prorelaxin could be converted to the 6-kDa relaxin by limited digestion with trypsin. Trypsin was shown to cleave at the carboxyl side of Arg29 and Arg137 residues of the recombinant prorelaxin, producing the des-ArgA1-B29-relaxin, and degrade the 13-kDa connecting peptide into small peptides. Both the recombinant prorelaxin and converted relaxin were found to be biologically active in an in vitro bioassay for relaxin.

Original languageEnglish (US)
Pages (from-to)579-585
Number of pages7
JournalArchives of Biochemistry and Biophysics
Volume294
Issue number2
DOIs
StatePublished - May 1 1992

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Escherichia coli
Purification
Relaxin
Swine
Trypsin
Nalidixic Acid
Glutathione Disulfide
prorelaxin
Bioassay
C-Peptide
Guanidine
Reverse-Phase Chromatography
Chromatography
Cellulose
Biological Assay
Glutathione
Sequence Analysis
Digestion
Plasmids
Nucleotides

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Purification and characterization of recombinant porcine prorelaxin expressed in Escherichia coli. / Reddy, G. Kesava; Gunwar, Sripad; Green, Carla B.; Fei, David T W; Chen, Anthony B.; Kwok, Simon C M.

In: Archives of Biochemistry and Biophysics, Vol. 294, No. 2, 01.05.1992, p. 579-585.

Research output: Contribution to journalArticle

Reddy, G. Kesava ; Gunwar, Sripad ; Green, Carla B. ; Fei, David T W ; Chen, Anthony B. ; Kwok, Simon C M. / Purification and characterization of recombinant porcine prorelaxin expressed in Escherichia coli. In: Archives of Biochemistry and Biophysics. 1992 ; Vol. 294, No. 2. pp. 579-585.
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