Purification and characterization of the GalNAc-4-sulfotransferase responsible for sulfation of GalNAcβ1,4GlcNAc-bearing oligosaccharides

L. V. Hooper, O. Hindsgaul, J. U. Baenziger

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Abstract

The pituitary glycoprotein hormone lutropin is characterized by its pulsatile appearance in the bloodstream which is important for the expression of its biological activity in the ovary. We have previously shown that lutropin bears unique Asn-linked oligosaccharides terminating with GalNAc-4- SO4 which allow the hormone to be rapidly cleared from the bloodstream via a specific receptor in the liver, thus contributing to its pulsatile appearance in the circulation. Furthermore, we have found that carbonic anhydrase VI, synthesized by the submaxillary gland and secreted into the saliva, also bears Asn-linked oligosaccharides terminating with GalNAc-4-SO4, suggesting that this unique sulfated structure mediates other biological functions in addition to rapid clearance from the circulation. We report here the purification of a GalNAc-4-sulfotransferase which transfers sulfate to terminal β1,4-linked GalNAc on Ash-linked oligosaccharides. We show that the purified submaxillary gland enzyme has kinetic parameters identical to the pituitary enzyme, indicating that the same sulfotransferase is responsible for the sulfation of lutropin oligosaccharides in pituitary and carbonic anhydrase VI oligosaccharides in submaxillary gland. This GalNAc-4- sulfotransferase has an apparent molecular mass of 128 kDa and can be specifically photoaffinity radiolabeled with 3',5'-ADP, a competitive inhibitor of sulfotransferase activity. The acceptor specificity of this GalNAc-4-sulfotransferase indicates that it is able to transfer sulfate to terminal GalNAcβ1,4GlcNAc on both N- and O-glycosidically linked oligosaccharides, suggesting that this enzyme is also responsible for the sulfation of O-linked glycans on proopiomelanocortin.

Original languageEnglish (US)
Pages (from-to)16327-16332
Number of pages6
JournalJournal of Biological Chemistry
Volume270
Issue number27
DOIs
StatePublished - 1995

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Bearings (structural)
Oligosaccharides
Purification
Submandibular Gland
Luteinizing Hormone
Sulfotransferases
Ashes
Sulfates
Enzymes
Hormones
Enzyme kinetics
Pro-Opiomelanocortin
Pituitary Hormones
Molecular mass
Bioactivity
Saliva
Kinetic parameters
Liver
Polysaccharides
N-acetylgalactosamine-4-sulfotransferase

ASJC Scopus subject areas

  • Biochemistry

Cite this

Purification and characterization of the GalNAc-4-sulfotransferase responsible for sulfation of GalNAcβ1,4GlcNAc-bearing oligosaccharides. / Hooper, L. V.; Hindsgaul, O.; Baenziger, J. U.

In: Journal of Biological Chemistry, Vol. 270, No. 27, 1995, p. 16327-16332.

Research output: Contribution to journalArticle

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