Purification and functional analysis of Ric-8A: A guanine nucleotide exchange factor for G-protein α subunits

Gregory G. Tall, Alfred G. Gilman

Research output: Contribution to journalArticle

17 Scopus citations

Abstract

Ric-8A (synembryn) has been shown to accelerate the in vitro guanine nucleotide exchange activities of most G-protein α subunits (with the exception of Gαs). Methods are presented in this article for the purification of Ric-8A and functional analysis of the effects Ric-8A has on G-protein α subunit guanine nucleotide-binding activities. The use of Ric-8A to prepare GTPγS-Gα and nucleotide-free Gα (in complex with Ric-8A) is described.

Original languageEnglish (US)
Pages (from-to)377-388
Number of pages12
JournalMethods in Enzymology
Volume390
DOIs
Publication statusPublished - 2004

    Fingerprint

ASJC Scopus subject areas

  • Biochemistry

Cite this