Abstract
Ric-8A (synembryn) has been shown to accelerate the in vitro guanine nucleotide exchange activities of most G-protein α subunits (with the exception of Gαs). Methods are presented in this article for the purification of Ric-8A and functional analysis of the effects Ric-8A has on G-protein α subunit guanine nucleotide-binding activities. The use of Ric-8A to prepare GTPγS-Gα and nucleotide-free Gα (in complex with Ric-8A) is described.
Original language | English (US) |
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Pages (from-to) | 377-388 |
Number of pages | 12 |
Journal | Methods in Enzymology |
Volume | 390 |
DOIs | |
State | Published - 2004 |
ASJC Scopus subject areas
- Biochemistry