Purification and properties of a neutral protease from rat liver chromatin

Ming Ta Chong, William T. Garrard, James Bonner

Research output: Contribution to journalArticle

87 Citations (Scopus)

Abstract

Rat liver chromatin contains a neutral protease with a marked preference for chromosomal proteins as substrate. The enzyme has been purified about 700-fold. It has a molecular weight of 200,000 with two subunits. It is inhibited by phenylmethanesulfonyl fluoride and diisopropyl fluorophosphate. The enzyme requires divalent ions as activators. The isolated enzyme appears to be similar to that responsible for the endogenous degradation of chromosomal proteins. The susceptibility of the five histone fractions to proteolysis is dependent upon whether the histones are complexed with DNA. In the intact nucleohistone, four major histones are rather resistant to proteolytic attack while histone I is rapidly attacked. If histones are freed from DNA, all the histone molecules are attacked at about the same rate except histone I, which is degraded more slowly than the other histones.

Original languageEnglish (US)
Pages (from-to)5128-5134
Number of pages7
JournalBiochemistry
Volume13
Issue number25
StatePublished - 1974

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Liver
Histones
Chromatin
Purification
Rats
Peptide Hydrolases
fluorophosphate
Proteolysis
Enzymes
Phenylmethylsulfonyl Fluoride
DNA
Proteins
Molecular Weight
Molecular weight
Ions
Degradation
Molecules
Substrates

ASJC Scopus subject areas

  • Biochemistry

Cite this

Chong, M. T., Garrard, W. T., & Bonner, J. (1974). Purification and properties of a neutral protease from rat liver chromatin. Biochemistry, 13(25), 5128-5134.

Purification and properties of a neutral protease from rat liver chromatin. / Chong, Ming Ta; Garrard, William T.; Bonner, James.

In: Biochemistry, Vol. 13, No. 25, 1974, p. 5128-5134.

Research output: Contribution to journalArticle

Chong, MT, Garrard, WT & Bonner, J 1974, 'Purification and properties of a neutral protease from rat liver chromatin', Biochemistry, vol. 13, no. 25, pp. 5128-5134.
Chong MT, Garrard WT, Bonner J. Purification and properties of a neutral protease from rat liver chromatin. Biochemistry. 1974;13(25):5128-5134.
Chong, Ming Ta ; Garrard, William T. ; Bonner, James. / Purification and properties of a neutral protease from rat liver chromatin. In: Biochemistry. 1974 ; Vol. 13, No. 25. pp. 5128-5134.
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