Abstract
A method for the preparation of immunotoxins (ITs) containing one and two molecules of deglycosylated ricin A chain (dgA) bound to each molecule of anti-human CD22 mouse IgG1 monoclonal antibody is described. The cross-linking of antibody and dgA molecules was accomplished with N-succinimidyl-oxycarbonyl-α-methyl-(2-pyridyldithio). The two ITs were purified by successive chromatographies on Blue-Sepharose, Sephacryl S-200, Blue-Sepharose with NaCl gradient and Sephacryl S-300. The IT with two dgAs was seven-fold more cytotoxic to CD22+ Daudi cells than that containing one molecule of dgA.
Original language | English (US) |
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Pages (from-to) | 117-122 |
Number of pages | 6 |
Journal | Journal of Immunological Methods |
Volume | 166 |
Issue number | 1 |
DOIs | |
State | Published - Nov 5 1993 |
Keywords
- (Antibody)
- Immunotoxin
- Ricin A chain
ASJC Scopus subject areas
- Immunology and Allergy
- Immunology