Purification and properties of immunotoxins containing one vs. two deglycosylated ricin A chains

Victor Ghetie, Eric Swindell, Jonathan W. Uhr, Ellen S. Vitetta

Research output: Contribution to journalArticle

23 Scopus citations

Abstract

A method for the preparation of immunotoxins (ITs) containing one and two molecules of deglycosylated ricin A chain (dgA) bound to each molecule of anti-human CD22 mouse IgG1 monoclonal antibody is described. The cross-linking of antibody and dgA molecules was accomplished with N-succinimidyl-oxycarbonyl-α-methyl-(2-pyridyldithio). The two ITs were purified by successive chromatographies on Blue-Sepharose, Sephacryl S-200, Blue-Sepharose with NaCl gradient and Sephacryl S-300. The IT with two dgAs was seven-fold more cytotoxic to CD22+ Daudi cells than that containing one molecule of dgA.

Original languageEnglish (US)
Pages (from-to)117-122
Number of pages6
JournalJournal of Immunological Methods
Volume166
Issue number1
DOIs
StatePublished - Nov 5 1993

Keywords

  • (Antibody)
  • Immunotoxin
  • Ricin A chain

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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