Purification and reconstitution of sterol transfer by native mouse ABCG5 and ABCG8

Jin Wang, Da Wei Zhang, Ying Lei, Fang Xu, Jonathan C. Cohen, Helen H. Hobbs, Xiao Song Xie

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

ABCG5 (G5) and ABCG8 (G8) are ATP-binding cassette half-transporters that limit intestinal uptake and promote biliary secretion of neutral sterols. Here, we describe the purification of endogenous G5G8 from mouse liver to near homogeneity. We incorporated the native proteins into membrane vesicles and reconstituted sterol transfer. Native gel electrophoresis, density-gradient ultracentrifugation, and chemical cross-linking studies indicated that the functional native complex is a heterodimer. No higher order oligomeric forms were observed at any stage in the catalytic cycle. Sterol transfer activity by purified native G5G8 was stable, stereospecific, and selective. We also report that G5 but not G8 is S-palmitoylated and that palmitoylation is not essential for dimerization, trafficking, or biliary sterol secretion. Both G5 and G8 have short but highly conserved cytoplasmic tails. The functional roles of these C-terminal regions were examined using an in vivo functional assay.

Original languageEnglish (US)
Pages (from-to)5194-5204
Number of pages11
JournalBiochemistry
Volume47
Issue number18
DOIs
StatePublished - May 6 2008

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Sterols
Purification
Lipoylation
ATP-Binding Cassette Transporters
Dimerization
Ultracentrifugation
Electrophoresis
Liver
Tail
Assays
Membrane Proteins
Adenosine Triphosphate
Gels
Membranes
Proteins

ASJC Scopus subject areas

  • Biochemistry

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Purification and reconstitution of sterol transfer by native mouse ABCG5 and ABCG8. / Wang, Jin; Zhang, Da Wei; Lei, Ying; Xu, Fang; Cohen, Jonathan C.; Hobbs, Helen H.; Xie, Xiao Song.

In: Biochemistry, Vol. 47, No. 18, 06.05.2008, p. 5194-5204.

Research output: Contribution to journalArticle

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