Purification, crystallization and preliminary crystallographic analysis of the non-Pfam protein AF1514 from Archeoglobus fulgidus DSM 4304

Pazilat Bahti, Shunmei Chen, Yang Li, Neil Shaw, Xuejun Zhang, Min Zhang, Chongyun Cheng, Gaojie Song, Jie Yin, Hua Zhang, Dongsheng Che, Abdulla Abbas, Hao Xu, Bi Cheng Wang, Zhi Jie Liu

Research output: Contribution to journalArticlepeer-review

Abstract

A 10.5 kDa non-Pfam hypothetical protein, AF1514, from the hyperthermophilic archaeon Archeoglobus fulgidus has been overexpressed in Escherichia coli, purified and crystallized using the hanging-drop vapour-diffusion method. The crystals diffracted X-rays to 2.09 Å resolution and a data set was collected at 100 K using Cu Kα radiation from a rotating-anode X-ray source. The crystals belong to space group P4 1212 or P43212, with unit-cell parameters a = b = 49.27, c = 106.61 Å. The calculated Matthews coefficient was 3.16 Å3 Da-1, suggesting the presence of one molecule in the asymmetric unit.

Original languageEnglish (US)
Pages (from-to)91-93
Number of pages3
JournalActa Crystallographica Section F: Structural Biology and Crystallization Communications
Volume64
Issue number2
DOIs
StatePublished - 2008

Keywords

  • Non-Pfam proteins
  • Reductive methylation

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Genetics
  • Condensed Matter Physics

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