Purification, crystallization and preliminary crystallographic analysis of the non-Pfam protein AF1514 from Archeoglobus fulgidus DSM 4304

Pazilat Bahti, Shunmei Chen, Yang Li, Neil Shaw, Xuejun Zhang, Min Zhang, Chongyun Cheng, Gaojie Song, Jie Yin, Hua Zhang, Dongsheng Che, Abdulla Abbas, Hao Xu, Bi Cheng Wang, Zhi Jie Liu

Research output: Contribution to journalArticle

Abstract

A 10.5 kDa non-Pfam hypothetical protein, AF1514, from the hyperthermophilic archaeon Archeoglobus fulgidus has been overexpressed in Escherichia coli, purified and crystallized using the hanging-drop vapour-diffusion method. The crystals diffracted X-rays to 2.09 Å resolution and a data set was collected at 100 K using Cu Kα radiation from a rotating-anode X-ray source. The crystals belong to space group P4 1212 or P43212, with unit-cell parameters a = b = 49.27, c = 106.61 Å. The calculated Matthews coefficient was 3.16 Å3 Da-1, suggesting the presence of one molecule in the asymmetric unit.

Original languageEnglish (US)
Pages (from-to)91-93
Number of pages3
JournalActa Crystallographica Section F: Structural Biology and Crystallization Communications
Volume64
Issue number2
DOIs
StatePublished - Mar 12 2008
Externally publishedYes

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Crystallization
purification
Purification
X-Rays
crystallization
proteins
X rays
Crystals
Archaea
Escherichia
Escherichia coli
crystals
Anodes
Electrodes
Proteins
anodes
x rays
Vapors
vapors
Radiation

Keywords

  • Non-Pfam proteins
  • Reductive methylation

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Genetics
  • Condensed Matter Physics

Cite this

Purification, crystallization and preliminary crystallographic analysis of the non-Pfam protein AF1514 from Archeoglobus fulgidus DSM 4304. / Bahti, Pazilat; Chen, Shunmei; Li, Yang; Shaw, Neil; Zhang, Xuejun; Zhang, Min; Cheng, Chongyun; Song, Gaojie; Yin, Jie; Zhang, Hua; Che, Dongsheng; Abbas, Abdulla; Xu, Hao; Wang, Bi Cheng; Liu, Zhi Jie.

In: Acta Crystallographica Section F: Structural Biology and Crystallization Communications, Vol. 64, No. 2, 12.03.2008, p. 91-93.

Research output: Contribution to journalArticle

Bahti, Pazilat ; Chen, Shunmei ; Li, Yang ; Shaw, Neil ; Zhang, Xuejun ; Zhang, Min ; Cheng, Chongyun ; Song, Gaojie ; Yin, Jie ; Zhang, Hua ; Che, Dongsheng ; Abbas, Abdulla ; Xu, Hao ; Wang, Bi Cheng ; Liu, Zhi Jie. / Purification, crystallization and preliminary crystallographic analysis of the non-Pfam protein AF1514 from Archeoglobus fulgidus DSM 4304. In: Acta Crystallographica Section F: Structural Biology and Crystallization Communications. 2008 ; Vol. 64, No. 2. pp. 91-93.
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AU - Bahti, Pazilat

AU - Chen, Shunmei

AU - Li, Yang

AU - Shaw, Neil

AU - Zhang, Xuejun

AU - Zhang, Min

AU - Cheng, Chongyun

AU - Song, Gaojie

AU - Yin, Jie

AU - Zhang, Hua

AU - Che, Dongsheng

AU - Abbas, Abdulla

AU - Xu, Hao

AU - Wang, Bi Cheng

AU - Liu, Zhi Jie

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AB - A 10.5 kDa non-Pfam hypothetical protein, AF1514, from the hyperthermophilic archaeon Archeoglobus fulgidus has been overexpressed in Escherichia coli, purified and crystallized using the hanging-drop vapour-diffusion method. The crystals diffracted X-rays to 2.09 Å resolution and a data set was collected at 100 K using Cu Kα radiation from a rotating-anode X-ray source. The crystals belong to space group P4 1212 or P43212, with unit-cell parameters a = b = 49.27, c = 106.61 Å. The calculated Matthews coefficient was 3.16 Å3 Da-1, suggesting the presence of one molecule in the asymmetric unit.

KW - Non-Pfam proteins

KW - Reductive methylation

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