Purification, crystallization and preliminary X-ray studies of GMP reductase 2 from human

Chao Neng Ji; Gang Ying; Ying Feng Deng; Shu Chen; Wen Hong Zhang; Guang Shu; Yi Xie; Yu Min Mao

doi:10.1107/S0907444903008588

Purification, crystallization and preliminary X-ray studies of GMP reductase 2 from human

Chao Neng Ji, Gang Ying, Ying Feng Deng, Shu Chen, Wen Hong Zhang, Guang Shu, Yi Xie, Yu Min Mao

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Abstract

GMP reductase 2 from human has been expressed in Escherichia coli, purified and crystallized. The crystals belong to space group P3₂21, with unit-cell parameters a = b = 110.6, c = 209.8 Å, α = β = 90, γ = 120°. Diffraction data were collected to 3.0 Å with a completeness of 100% (100% for the last shell), an R_merge value of 0.089 (0.189) and an I/σ(I) value of 7.3 (3.2).

Original language	English (US)
Pages (from-to)	1109-1110
Number of pages	2
Journal	Acta Crystallographica - Section D Biological Crystallography
Volume	59
Issue number	6
DOIs	https://doi.org/10.1107/S0907444903008588
State	Published - Jun 1 2003

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Structural Biology

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title = "Purification, crystallization and preliminary X-ray studies of GMP reductase 2 from human",

abstract = "GMP reductase 2 from human has been expressed in Escherichia coli, purified and crystallized. The crystals belong to space group P3221, with unit-cell parameters a = b = 110.6, c = 209.8 {\AA}, α = β = 90, γ = 120°. Diffraction data were collected to 3.0 {\AA} with a completeness of 100% (100% for the last shell), an Rmerge value of 0.089 (0.189) and an I/σ(I) value of 7.3 (3.2).",

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T1 - Purification, crystallization and preliminary X-ray studies of GMP reductase 2 from human

AU - Ji, Chao Neng

AU - Ying, Gang

AU - Deng, Ying Feng

AU - Chen, Shu

AU - Zhang, Wen Hong

AU - Shu, Guang

AU - Xie, Yi

AU - Mao, Yu Min

PY - 2003/6/1

Y1 - 2003/6/1

N2 - GMP reductase 2 from human has been expressed in Escherichia coli, purified and crystallized. The crystals belong to space group P3221, with unit-cell parameters a = b = 110.6, c = 209.8 Å, α = β = 90, γ = 120°. Diffraction data were collected to 3.0 Å with a completeness of 100% (100% for the last shell), an Rmerge value of 0.089 (0.189) and an I/σ(I) value of 7.3 (3.2).

AB - GMP reductase 2 from human has been expressed in Escherichia coli, purified and crystallized. The crystals belong to space group P3221, with unit-cell parameters a = b = 110.6, c = 209.8 Å, α = β = 90, γ = 120°. Diffraction data were collected to 3.0 Å with a completeness of 100% (100% for the last shell), an Rmerge value of 0.089 (0.189) and an I/σ(I) value of 7.3 (3.2).

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Purification, crystallization and preliminary X-ray studies of GMP reductase 2 from human

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