Purification of a novel RECQL5-SWI/SNF-RNAPII super complex

Guangjin Zhou, Yifei Liu, Shwu Yuan Wu, Feng Tie, Hua Lou, Cheng Ming Chiang, Guangbin Luo

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

RecQ helicases are members of an evolutionarily conserved family of DNA helicases. They are homologous to the RecQ helicase of E. coli, the founding member of the family. These enzymes include gene products of diseasecausing genes in Bloom, Werner, and Rothmund-Thomson syndrome. To date, these proteins have been implicated in many aspects of DNA metabolism, including DNA replication, repair, and recombination. We reported here that RECQL5, a newer member of the human RecQ helicase family, physically interacts with SWI/SNF complex and RNAPII core complex within the context of a super complex. RECQL5 was detected in the RNAPII holoenzyme but not in purified RNAPII core complex. Together, these data link RECQL5 to the assembly of the RNAPII transcription machinery and suggest that this helicase may have a regulatory role in RNAPII transcription or an RNAPII-related process or processes.

Original languageEnglish (US)
Pages (from-to)101-111
Number of pages11
JournalInternational Journal of Biochemistry and Molecular Biology
Volume1
Issue number1
StatePublished - 2010

Fingerprint

RecQ Helicases
Purification
Transcription
Rothmund-Thomson Syndrome
Genes
DNA Helicases
Holoenzymes
DNA
DNA Replication
Metabolism
DNA Repair
Escherichia coli
Genetic Recombination
Machinery
Repair
Enzymes
Proteins

Keywords

  • DNA helicases
  • E. Coli
  • Purification
  • RecQ helicases
  • RECQL5-SWI/SNF-RNAPII super complex

ASJC Scopus subject areas

  • Molecular Biology
  • Biochemistry

Cite this

Purification of a novel RECQL5-SWI/SNF-RNAPII super complex. / Zhou, Guangjin; Liu, Yifei; Wu, Shwu Yuan; Tie, Feng; Lou, Hua; Chiang, Cheng Ming; Luo, Guangbin.

In: International Journal of Biochemistry and Molecular Biology, Vol. 1, No. 1, 2010, p. 101-111.

Research output: Contribution to journalArticle

Zhou, Guangjin ; Liu, Yifei ; Wu, Shwu Yuan ; Tie, Feng ; Lou, Hua ; Chiang, Cheng Ming ; Luo, Guangbin. / Purification of a novel RECQL5-SWI/SNF-RNAPII super complex. In: International Journal of Biochemistry and Molecular Biology. 2010 ; Vol. 1, No. 1. pp. 101-111.
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AU - Chiang, Cheng Ming

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