Purification of immunotoxins containing ricin A-chain and abrin A-chain using Blue Sepharose CL-6B

Phillip P. Knowles; Philip E. Thorpe

doi:10.1016/0003-2697(87)90073-X

Purification of immunotoxins containing ricin A-chain and abrin A-chain using Blue Sepharose CL-6B

Phillip P. Knowles, Philip E. Thorpe

Pharmacology

Research output: Contribution to journal › Article › peer-review

78 Scopus citations

Abstract

We describe a method for separating antibody from immunotoxins by affinity chromatography on Cibacron blue F3GA coupled to Sepharose (Blue Sepharose). The antibody did not bind to the gel. The immunotoxins were bound by their ricin A-chain or abrin A-chain moiety and could be recovered in high yield and purity using mild elution conditions. The method is suitable for the large-scale purification of immunotoxins.

Original language	English (US)
Pages (from-to)	440-443
Number of pages	4
Journal	Analytical Biochemistry
Volume	160
Issue number	2
DOIs	https://doi.org/10.1016/0003-2697(87)90073-X
State	Published - Feb 1 1987

Keywords

Cibacron blue F3GA
abrin
affinity chromatography
immunotoxin
ricin

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/0003-2697(87)90073-X

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abstract = "We describe a method for separating antibody from immunotoxins by affinity chromatography on Cibacron blue F3GA coupled to Sepharose (Blue Sepharose). The antibody did not bind to the gel. The immunotoxins were bound by their ricin A-chain or abrin A-chain moiety and could be recovered in high yield and purity using mild elution conditions. The method is suitable for the large-scale purification of immunotoxins.",

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AU - Knowles, Phillip P.

AU - Thorpe, Philip E.

PY - 1987/2/1

Y1 - 1987/2/1

N2 - We describe a method for separating antibody from immunotoxins by affinity chromatography on Cibacron blue F3GA coupled to Sepharose (Blue Sepharose). The antibody did not bind to the gel. The immunotoxins were bound by their ricin A-chain or abrin A-chain moiety and could be recovered in high yield and purity using mild elution conditions. The method is suitable for the large-scale purification of immunotoxins.

AB - We describe a method for separating antibody from immunotoxins by affinity chromatography on Cibacron blue F3GA coupled to Sepharose (Blue Sepharose). The antibody did not bind to the gel. The immunotoxins were bound by their ricin A-chain or abrin A-chain moiety and could be recovered in high yield and purity using mild elution conditions. The method is suitable for the large-scale purification of immunotoxins.

KW - Cibacron blue F3GA

KW - abrin

KW - affinity chromatography

KW - immunotoxin

KW - ricin

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Purification of immunotoxins containing ricin A-chain and abrin A-chain using Blue Sepharose CL-6B

Abstract

Keywords

ASJC Scopus subject areas

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