Purification of immunotoxins containing ricin A-chain and abrin A-chain using Blue Sepharose CL-6B

Phillip P. Knowles, Philip E. Thorpe

Research output: Contribution to journalArticle

72 Scopus citations

Abstract

We describe a method for separating antibody from immunotoxins by affinity chromatography on Cibacron blue F3GA coupled to Sepharose (Blue Sepharose). The antibody did not bind to the gel. The immunotoxins were bound by their ricin A-chain or abrin A-chain moiety and could be recovered in high yield and purity using mild elution conditions. The method is suitable for the large-scale purification of immunotoxins.

Original languageEnglish (US)
Pages (from-to)440-443
Number of pages4
JournalAnalytical Biochemistry
Volume160
Issue number2
DOIs
StatePublished - Feb 1 1987

Keywords

  • Cibacron blue F3GA
  • abrin
  • affinity chromatography
  • immunotoxin
  • ricin

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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