Purification of native Arp2/3 complex from bovine thymus

Lynda K. Doolittle, Michael K. Rosen, Shae B. Padrick

Research output: Chapter in Book/Report/Conference proceedingChapter

4 Scopus citations

Abstract

The Arp2/3 complex is an actin filament nucleator involved in cell motility and vesicle trafficking. Owing to the role the complex plays in important and fundamental cell biological processes, the purified complex is used in biochemical assays, reconstituted motility assays, and structural biology. As this is a eukaryotic complex assembled from seven polypeptides, the complex is purified from eukaryotic sources. Described here is a detailed method for purification of the complex from a mammalian tissue, bovine thymus.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages231-250
Number of pages20
Volume1046
ISBN (Print)9781627035378
DOIs
StatePublished - 2013

Publication series

NameMethods in Molecular Biology
Volume1046
ISSN (Print)10643745

Keywords

  • Actin nucleation factor
  • Arp2/3 complex
  • Biochemical purification
  • Detailed protocol
  • Endogenous source

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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    Doolittle, L. K., Rosen, M. K., & Padrick, S. B. (2013). Purification of native Arp2/3 complex from bovine thymus. In Methods in Molecular Biology (Vol. 1046, pp. 231-250). (Methods in Molecular Biology; Vol. 1046). Humana Press Inc.. https://doi.org/10.1007/978-1-62703-538-5_14