Purification of oxysterol binding protein from hamster liver cytosol

P. A. Dawson, D. R. Van Der Westhuyzen, J. L. Goldstein, M. S. Brown

Research output: Contribution to journalArticle

91 Citations (Scopus)

Abstract

We have purified to apparent homogeneity an oxysterol binding protein from cytosol of hamster livers. This protein, which corresponds to the protein described by Taylor and Kandutsch (Taylor, F.R., and Kandutsch, A. (1985) Chem. Phys. Lipids 38, 187-194), binds oxysterols such as 25-hydroxycholesterol but does not bind cholesterol or steroid hormones in vitro. It may participate in the feedback repression of enzymes of cholesterol biosynthesis and the low density lipoprotein receptor. The protein was purified more than 40,000-fold with a series of ion exchange chromatography steps. The final preparation contained a doublet of peptides with molecular weights (M(r)) of 101,000 and 96,000, as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. These components formed a complex that migrated on gel filtration with an apparent M(r) of 280,000 in the absence or presence of 25-hydroxycholesterol. The amino acid sequence of a tryptic peptide from this protein complex was obtained, and a monoclonal antipeptide antibody was prepared. The antibody stained both the 101,000- and 96,000-Da proteins on immunoblots, suggesting that these two components are closely related and that one may be a modified or proteolyzed form of the other. With the purified protein now available, it should become possible to determine the role, if any, that this protein plays in the regulation of intracellular cholesterol metabolism.

Original languageEnglish (US)
Pages (from-to)9046-9052
Number of pages7
JournalJournal of Biological Chemistry
Volume264
Issue number15
StatePublished - 1989

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Cricetinae
Liver
Cytosol
Purification
Proteins
Cholesterol
Enzyme Repression
Steroid hormones
Peptides
Monoclonal antibodies
LDL Receptors
Biosynthesis
Ion Exchange Chromatography
Chromatography
oxysterol binding protein
Electrophoresis
Metabolism
Sodium Dodecyl Sulfate
Gel Chromatography
Polyacrylamide Gel Electrophoresis

ASJC Scopus subject areas

  • Biochemistry

Cite this

Purification of oxysterol binding protein from hamster liver cytosol. / Dawson, P. A.; Van Der Westhuyzen, D. R.; Goldstein, J. L.; Brown, M. S.

In: Journal of Biological Chemistry, Vol. 264, No. 15, 1989, p. 9046-9052.

Research output: Contribution to journalArticle

Dawson, P. A. ; Van Der Westhuyzen, D. R. ; Goldstein, J. L. ; Brown, M. S. / Purification of oxysterol binding protein from hamster liver cytosol. In: Journal of Biological Chemistry. 1989 ; Vol. 264, No. 15. pp. 9046-9052.
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