Purification of solid-phase synthesized peptides on the coil planet centrifuge

M. Knight, A. M. Kask, C. A. Tamminga

Research output: Contribution to journalArticle

16 Scopus citations

Abstract

The analytical flow-through coil planet centrifuge, an instrument for countercurrent chromatography, performs the preparative purification of synthetic peptides. Various two-phase solvent systems have been tried with either phase mobile to purify many synthesized peptides. A series of N-terminal fragment peptides of cholecystokinin octapeptide (CCK 26–33) were synthesized by solid-phase techniques and purified on the coil planet centrifuge. The peptides were sulfated and chromatographed again. For hydrophobic peptides, purification is effected in solvent systems with a mobile aqueous phase. The n-butanol, acetic acid and water system (4:1:5 by volume) with the lower phase mobile was utilized. For sulfated peptides, the neutral system, 0.2 M ammonium acetate and n-butanol was generally applied.

Original languageEnglish (US)
Pages (from-to)351-362
Number of pages12
JournalJournal of Liquid Chromatography
Volume7
Issue number2
DOIs
StatePublished - Feb 1 1984

ASJC Scopus subject areas

  • Molecular Medicine

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