PURIFICATION OF SOLID-PHASE SYNTHESIZED PEPTIDES ON THE COIL PLANET CENTRIFUGE.

M. Knight, A. M. Kask, C. A. Tamminga

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

The analytical flow-through coil planet centrifuge, an instrument for countercurrent chromatography, performs the preparative purification of synthetic peptides. Various two-phase solvent systems have been tried with either phase mobile to purify many synthesized peptides. A series of N-terminal fragment peptides of cholecystokinin octapeptide (CCK 26-33) were synthesized by solid-phase techniques and purified on the coil planet centrifuge. The peptides were sulfated and chromatographed again. For hydrophobic peptides, purification is effected in solvent systems with a mobile aqueous phase.

Original languageEnglish (US)
Pages (from-to)351-362
Number of pages12
JournalJournal of Liquid Chromatography
Volume7
Issue number2
StatePublished - Feb 1984

Fingerprint

Planets
Centrifuges
Purification
Peptides
Countercurrent Distribution
Sincalide
Peptide Fragments
Chromatography

ASJC Scopus subject areas

  • Molecular Medicine

Cite this

PURIFICATION OF SOLID-PHASE SYNTHESIZED PEPTIDES ON THE COIL PLANET CENTRIFUGE. / Knight, M.; Kask, A. M.; Tamminga, C. A.

In: Journal of Liquid Chromatography, Vol. 7, No. 2, 02.1984, p. 351-362.

Research output: Contribution to journalArticle

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