Puromycin-induced argyrophilia as an alternative to golgi methods: Reliable impregnations of hypothalamic cell groups following lateral ventricular injections in rats

Steven Warach, William E. Armstrong, Glenn I. Hatton

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

Parenchymal injections of puromycin were known to induce Golgi-like silver impregnations upon treatment with the cupric-silver method. We have found lateral ventricular injections (5 μl, 0.1 M) also effective. Such injections consistently led to detailed Golgi-like profiles of cells in a number of hypothalamic regions including one, the paraventricular nucleus, which traditionally is quite recalcitrant to Golgi methods. It is concluded that this technique is a reliable alternative to Golgi methods for the study of many hypothalamic regions.

Original languageEnglish (US)
Pages (from-to)223-228
Number of pages6
JournalNeuroscience Letters
Volume23
Issue number3
DOIs
StatePublished - May 29 1981

ASJC Scopus subject areas

  • Neuroscience(all)

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