TY - JOUR
T1 - Puromycin-induced argyrophilia as an alternative to golgi methods
T2 - Reliable impregnations of hypothalamic cell groups following lateral ventricular injections in rats
AU - Warach, Steven
AU - Armstrong, William E.
AU - Hatton, Glenn I.
N1 - Funding Information:
We are grateful to Dr. R.C. Switzer for his encouragement and valuable advice on the use of the puromycin and.cupric-silver techniques. We also thank A.K. Salm for critically reading the manuscript and J. Harper for typing. This research was supported by NSF Graduate Fellowship to S.W., NIH Grant NS 01940 (NINCDS) to G.I.H,, and the Neuroscience Program of Michigan State Univelsity.
PY - 1981/5/29
Y1 - 1981/5/29
N2 - Parenchymal injections of puromycin were known to induce Golgi-like silver impregnations upon treatment with the cupric-silver method. We have found lateral ventricular injections (5 μl, 0.1 M) also effective. Such injections consistently led to detailed Golgi-like profiles of cells in a number of hypothalamic regions including one, the paraventricular nucleus, which traditionally is quite recalcitrant to Golgi methods. It is concluded that this technique is a reliable alternative to Golgi methods for the study of many hypothalamic regions.
AB - Parenchymal injections of puromycin were known to induce Golgi-like silver impregnations upon treatment with the cupric-silver method. We have found lateral ventricular injections (5 μl, 0.1 M) also effective. Such injections consistently led to detailed Golgi-like profiles of cells in a number of hypothalamic regions including one, the paraventricular nucleus, which traditionally is quite recalcitrant to Golgi methods. It is concluded that this technique is a reliable alternative to Golgi methods for the study of many hypothalamic regions.
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U2 - 10.1016/0304-3940(81)90001-X
DO - 10.1016/0304-3940(81)90001-X
M3 - Article
C2 - 6167908
AN - SCOPUS:0019870414
SN - 0304-3940
VL - 23
SP - 223
EP - 228
JO - Neuroscience letters
JF - Neuroscience letters
IS - 3
ER -