Quantification of cyclin A1 and glyceraldehyde-3-phosphate dehydrogenase expression in testicular biopsies of infertile patients by fluorescence real-time RT-PCR

Mark Schrader, Stuart Ravnik, Carsten Müller-Tidow, Markus Müller, Bernd Straub, Sven Diedrichs, Hubert Serve, Kurt Miller

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

The objective of this study was to establish a classification of meiotic disorders by detection of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and cyclin A1 mRNA in patients presenting with non-obstructive azoospermia. GAPDH and cyclin A1 expression levels were detected in 37 histologically classified testicular tissue specimens by one-line RT-PCR. Levels of cyclin A1 expression (NCyclinA1) were high in tissue specimens with full spermatogenesis [3.519 ± 3.141 (mean ± SD)] but only minimal in those without haploid germ cells. A lack of cyclin A1 expression was seen in most sertoli-cell-only syndrome (SCOS) specimens. The criteria for an ideal internal standard were only partially met by GAPDH, as we detected decreased expression in tissue samples with maturation arrest [(268.2 ± 106.2 relative gene expression (mean ± SD)] and SCOS (201.7 ± 95.2) compared to those with normal histological findings (325.1 ± 129.3). It was concluded that the level of cyclin A1 mRNA expression predicts meiotic disorders during spermatogenesis. GAPDH is not an ideal internal standard for specific gene expression in testicular tissue specimens.

Original languageEnglish (US)
Pages (from-to)202-209
Number of pages8
JournalInternational Journal of Andrology
Volume25
Issue number4
DOIs
StatePublished - Jul 27 2002

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Keywords

  • Cyclin A1
  • Cyclin-dependent kinase
  • Male fertility
  • Spermatogenesis

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Reproductive Medicine
  • Urology

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