TY - JOUR
T1 - Quantification of endometriotic lesions in a murine model by fluorimetric and morphometric analyses
AU - Defrère, Sylvie
AU - Van Langendonckt, Anne
AU - Ramos, Reinaldo González
AU - Jouret, Mathieu
AU - Mettlen, Marcel
AU - Donnez, Jacques
N1 - Funding Information:
The authors thank Philippe Befahy, MD, Marie-Madeleine Dolmans, MD, Belen Martínez, VMD, PhD, Department of Gynaecology, for their assistance in the handling of mice and Mira Hryniuk, BA, for reviewing the manuscript. We also thank the Department of Anatomo-pathology for endometriotic lesion embedding and haematoxylin–eosin staining. The present study was supported by grant no. 3.4552.00 from the Fonds National de la Recherche Scientifique de Belgique and from the Fonds Spécial de la Recherche de l’Université Catholique de Louvain.
PY - 2006/3
Y1 - 2006/3
N2 - Background: In animal models of endometriosis, the identification and quantification of lesions originating from human endometrium is often hampered by the small size of the implants and their embedding in murine tissue. The purpose of the present study was to develop two new methods of quantifying endometriosis-like lesions in a nude mouse model: fluorimetry and morphometry. Methods: Human menstrual endometrium was labelled using a fluorescent tracker, carboxyfluorescein diacetate, succinimidyl ester (CFDA-SE), and transplanted into the pelvic cavity of mice by injection through the peritoneum after performing a cutaneous incision. After 5 days, lesions were recovered by laparotomy. The fluorescence of the recovered endometriotic lesions was measured. Endometrial stroma and glands were immunostained in lesion sections with anti-CD10 and anti-CK22 antibodies, and their surface area was evaluated by morphometric analysis. Results: Fluorescent labelling allows identification of lesions not visible macroscopically. A good correlation was observed between fluorimetry and morphometry (r = 0.88) applied for lesion quantification. Conclusions: Fluorimetric evaluation combined with morphometric analysis of endometriosis-like lesions allows objective and reliable recording of endometriosis development in a nude mouse model. This quantification method could therefore be useful for future pharmacological and toxicological studies.
AB - Background: In animal models of endometriosis, the identification and quantification of lesions originating from human endometrium is often hampered by the small size of the implants and their embedding in murine tissue. The purpose of the present study was to develop two new methods of quantifying endometriosis-like lesions in a nude mouse model: fluorimetry and morphometry. Methods: Human menstrual endometrium was labelled using a fluorescent tracker, carboxyfluorescein diacetate, succinimidyl ester (CFDA-SE), and transplanted into the pelvic cavity of mice by injection through the peritoneum after performing a cutaneous incision. After 5 days, lesions were recovered by laparotomy. The fluorescence of the recovered endometriotic lesions was measured. Endometrial stroma and glands were immunostained in lesion sections with anti-CD10 and anti-CK22 antibodies, and their surface area was evaluated by morphometric analysis. Results: Fluorescent labelling allows identification of lesions not visible macroscopically. A good correlation was observed between fluorimetry and morphometry (r = 0.88) applied for lesion quantification. Conclusions: Fluorimetric evaluation combined with morphometric analysis of endometriosis-like lesions allows objective and reliable recording of endometriosis development in a nude mouse model. This quantification method could therefore be useful for future pharmacological and toxicological studies.
KW - Carboxyfluorescein diacetate, succinimidyl ester
KW - Endometriosis
KW - Lesion quantification
KW - Menstrual endometrium
KW - Nude mouse model
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U2 - 10.1093/humrep/dei387
DO - 10.1093/humrep/dei387
M3 - Article
C2 - 16293650
AN - SCOPUS:33644903232
SN - 0268-1161
VL - 21
SP - 810
EP - 817
JO - Human Reproduction
JF - Human Reproduction
IS - 3
ER -