Quantification of pelvic organ prolapse in mice: Vaginal protease activity precedes increased MOPQ scores in fibulin 5 knockout Mice 1

Cecilia K. Wieslander, David D. Rahn, Donald D. Mclntire, Jesús F. Acevedo, Peter G. Drewes, Hiromi Yanagisawa, R. Ann Word

Research output: Contribution to journalArticlepeer-review

22 Scopus citations

Abstract

Two mouse models of pelvic organ prolapse have been generated recently, both of which have null mutations in genes involved in elastic fiber synthesis and assembly (fibulin 5 and lysyl oxidase-like 1). Interestingly, although these mice exhibit elastinopathies early in life, pelvic organ prolapse does not develop until later in life. In this investigation we developed and validated a tool to quantify the severity of pelvic organ prolapse in mice, and we used this tool prospectively to study the role of fibulin 5, aging, and vaginal proteases in the development of pelvic organ prolapse. The results indicate that >90% of Fbln5 -/- mice develop prolapse by 6 mo of age, even in the absence of vaginal delivery, and that increased vaginal protease activity precedes the development of prolapse.

Original languageEnglish (US)
Pages (from-to)407-414
Number of pages8
JournalBiology of reproduction
Volume80
Issue number3
DOIs
StatePublished - Mar 2009

Keywords

  • Elastic fibers
  • Female reproductive tract
  • Matrix degradation
  • Matrix metalloproteases
  • Mmp2
  • Mmp9
  • Pelvic organ prolapse
  • Vagina
  • Zymography

ASJC Scopus subject areas

  • Reproductive Medicine
  • Cell Biology

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