Quantifying kinetics and dynamics of DNA repair proteins using raster-scan image correlation spectroscopy and fluorescence recovery after photobleaching

Salim Abdisalaam, Milan Poudel, David Chen, George Alexandrakis

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

DNA double-strand breaks (DSBs) are one of the most lethal DNA damage occurs in mammalian cells. Improper repair of DSBs may leads to mutations, chromosomal translocations, apoptosis, genetic instability [1, 2], increases the chance to cancer development [3] and immune deficiency [4]. In this study the confocal Raster scan Image Correlation Spectroscopy technique is used to study kinetics and dynamics of double stand break repair proteins after γ-irradiation of mammalian cells. Diffusion and binding constants were obtained by fitting with different physical models. Results were compared to ones obtained by creating high density DNA damage with a laser and subsequently performing Fluorescence Recovery after Photobleaching over the damage area. This work presents similarities and differences in double strand break repair response between γ-irradiation versus laser damage.

Original languageEnglish (US)
Title of host publicationOptics InfoBase Conference Papers
StatePublished - 2011
EventBio-Optics: Design and Application, BODA 2011 - Monterey, CA, United States
Duration: Apr 4 2011Apr 6 2011

Other

OtherBio-Optics: Design and Application, BODA 2011
CountryUnited States
CityMonterey, CA
Period4/4/114/6/11

Fingerprint

Photobleaching
strands
DNA
Repair
deoxyribonucleic acid
Fluorescence
recovery
Spectroscopy
damage
proteins
Proteins
Recovery
fluorescence
Kinetics
kinetics
Cells
Irradiation
spectroscopy
Laser damage
irradiation

ASJC Scopus subject areas

  • Instrumentation
  • Atomic and Molecular Physics, and Optics

Cite this

Abdisalaam, S., Poudel, M., Chen, D., & Alexandrakis, G. (2011). Quantifying kinetics and dynamics of DNA repair proteins using raster-scan image correlation spectroscopy and fluorescence recovery after photobleaching. In Optics InfoBase Conference Papers

Quantifying kinetics and dynamics of DNA repair proteins using raster-scan image correlation spectroscopy and fluorescence recovery after photobleaching. / Abdisalaam, Salim; Poudel, Milan; Chen, David; Alexandrakis, George.

Optics InfoBase Conference Papers. 2011.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abdisalaam, S, Poudel, M, Chen, D & Alexandrakis, G 2011, Quantifying kinetics and dynamics of DNA repair proteins using raster-scan image correlation spectroscopy and fluorescence recovery after photobleaching. in Optics InfoBase Conference Papers. Bio-Optics: Design and Application, BODA 2011, Monterey, CA, United States, 4/4/11.
Abdisalaam, Salim ; Poudel, Milan ; Chen, David ; Alexandrakis, George. / Quantifying kinetics and dynamics of DNA repair proteins using raster-scan image correlation spectroscopy and fluorescence recovery after photobleaching. Optics InfoBase Conference Papers. 2011.
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