Quantifying kinetics and dynamics of DNA repair proteins using Raster-Scan Image Correlation Spectroscopy and Fluorescence Recovery after Photobleaching

Salim Abdisalaam, Milan Poudel, David Chen, George Alexandrakis

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

DNA double-strand breaks (DSBs) are one of the most lethal DNA damage occurs in mammalian cells. Improper repair of DSBs may leads to mutations, chromosomal translocations, apoptosis, genetic instability [1, 2], increases the chance to cancer development [3] and immune deficiency [4]. In this study the confocal Raster scan Image Correlation Spectroscopy technique is used to study kinetics and dynamics of double stand break repair proteins after γ-irradiation of mammalian cells. Diffusion and binding constants were obtained by fitting with different physical models. Results were compared to ones obtained by creating high density DNA damage with a laser and subsequently performing Fluorescence Recovery after Photobleaching over the damage area. This work presents similarities and differences in double strand break repair response between γ-irradiation versus laser damage.

Original languageEnglish (US)
Title of host publicationOptics InfoBase Conference Papers
Publication statusPublished - 2011
EventOptical Trapping Applications, OTA 2011 - Monterey, CA, United States
Duration: Apr 4 2011Apr 6 2011

Other

OtherOptical Trapping Applications, OTA 2011
CountryUnited States
CityMonterey, CA
Period4/4/114/6/11

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ASJC Scopus subject areas

  • Instrumentation
  • Atomic and Molecular Physics, and Optics

Cite this

Abdisalaam, S., Poudel, M., Chen, D., & Alexandrakis, G. (2011). Quantifying kinetics and dynamics of DNA repair proteins using Raster-Scan Image Correlation Spectroscopy and Fluorescence Recovery after Photobleaching. In Optics InfoBase Conference Papers