Quantifying kinetics and dynamics of DNA repair proteins using raster-scan image correlation spectroscopy and fluorescence recovery after photobleaching

Salim Abdisalaam, Milan Poudel, David Chen, George Alexandrakis

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

DNA double-strand breaks (DSBs) are one of the most lethal DNA damage occurs in mammalian cells. Improper repair of DSBs may leads to mutations, chromosomal translocations, apoptosis, genetic instability [1, 2], increases the chance to cancer development [3] and immune deficiency [4]. In this study the confocal Raster scan Image Correlation Spectroscopy technique is used to study kinetics and dynamics of double stand break repair proteins after γ-irradiation of mammalian cells. Diffusion and binding constants were obtained by fitting with different physical models. Results were compared to ones obtained by creating high density DNA damage with a laser and subsequently performing Fluorescence Recovery after Photobleaching over the damage area. This work presents similarities and differences in double strand break repair response between γ-irradiation versus laser damage.

Original languageEnglish (US)
Title of host publicationOptics InfoBase Conference Papers
PublisherOptical Society of American (OSA)
ISBN (Print)9781557529091
StatePublished - 2011
EventOptical Molecular Probes, Imaging and Drug Delivery, OMP 2011 - Monterey, CA, United States
Duration: Apr 4 2011Apr 6 2011

Other

OtherOptical Molecular Probes, Imaging and Drug Delivery, OMP 2011
CountryUnited States
CityMonterey, CA
Period4/4/114/6/11

Fingerprint

Photobleaching
strands
DNA
Repair
deoxyribonucleic acid
Fluorescence
recovery
Spectroscopy
damage
proteins
Proteins
Recovery
fluorescence
Kinetics
kinetics
Cells
Irradiation
spectroscopy
Laser damage
irradiation

ASJC Scopus subject areas

  • Instrumentation
  • Atomic and Molecular Physics, and Optics

Cite this

Abdisalaam, S., Poudel, M., Chen, D., & Alexandrakis, G. (2011). Quantifying kinetics and dynamics of DNA repair proteins using raster-scan image correlation spectroscopy and fluorescence recovery after photobleaching. In Optics InfoBase Conference Papers Optical Society of American (OSA).

Quantifying kinetics and dynamics of DNA repair proteins using raster-scan image correlation spectroscopy and fluorescence recovery after photobleaching. / Abdisalaam, Salim; Poudel, Milan; Chen, David; Alexandrakis, George.

Optics InfoBase Conference Papers. Optical Society of American (OSA), 2011.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abdisalaam, S, Poudel, M, Chen, D & Alexandrakis, G 2011, Quantifying kinetics and dynamics of DNA repair proteins using raster-scan image correlation spectroscopy and fluorescence recovery after photobleaching. in Optics InfoBase Conference Papers. Optical Society of American (OSA), Optical Molecular Probes, Imaging and Drug Delivery, OMP 2011, Monterey, CA, United States, 4/4/11.
Abdisalaam S, Poudel M, Chen D, Alexandrakis G. Quantifying kinetics and dynamics of DNA repair proteins using raster-scan image correlation spectroscopy and fluorescence recovery after photobleaching. In Optics InfoBase Conference Papers. Optical Society of American (OSA). 2011
Abdisalaam, Salim ; Poudel, Milan ; Chen, David ; Alexandrakis, George. / Quantifying kinetics and dynamics of DNA repair proteins using raster-scan image correlation spectroscopy and fluorescence recovery after photobleaching. Optics InfoBase Conference Papers. Optical Society of American (OSA), 2011.
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