TY - GEN
T1 - Quantifying kinetics and dynamics of DNA repair proteins using raster-scan image correlation spectroscopy and fluorescence recovery after photobleaching
AU - Abdisalaam, Salim
AU - Poudel, Milan
AU - Chen, David
AU - Alexandrakis, George
PY - 2011
Y1 - 2011
N2 - DNA double-strand breaks (DSBs) are one of the most lethal DNA damage occurs in mammalian cells. Improper repair of DSBs may leads to mutations, chromosomal translocations, apoptosis, genetic instability [1, 2], increases the chance to cancer development [3] and immune deficiency [4]. In this study the confocal Raster scan Image Correlation Spectroscopy technique is used to study kinetics and dynamics of double stand break repair proteins after γ-irradiation of mammalian cells. Diffusion and binding constants were obtained by fitting with different physical models. Results were compared to ones obtained by creating high density DNA damage with a laser and subsequently performing Fluorescence Recovery after Photobleaching over the damage area. This work presents similarities and differences in double strand break repair response between γ-irradiation versus laser damage.
AB - DNA double-strand breaks (DSBs) are one of the most lethal DNA damage occurs in mammalian cells. Improper repair of DSBs may leads to mutations, chromosomal translocations, apoptosis, genetic instability [1, 2], increases the chance to cancer development [3] and immune deficiency [4]. In this study the confocal Raster scan Image Correlation Spectroscopy technique is used to study kinetics and dynamics of double stand break repair proteins after γ-irradiation of mammalian cells. Diffusion and binding constants were obtained by fitting with different physical models. Results were compared to ones obtained by creating high density DNA damage with a laser and subsequently performing Fluorescence Recovery after Photobleaching over the damage area. This work presents similarities and differences in double strand break repair response between γ-irradiation versus laser damage.
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M3 - Conference contribution
AN - SCOPUS:84893533967
SN - 9781557529091
T3 - Optics InfoBase Conference Papers
BT - Bio-Optics
T2 - Bio-Optics: Design and Application, BODA 2011
Y2 - 4 April 2011 through 6 April 2011
ER -