Quantifying tracer levels of 2H2O enrichment from microliter amounts of plasma and urine by 2H NMR

John G. Jones; Matthew Merritt; Craig Malloy

doi:10.1002/1522-2594(200101)45:1<156::AID-MRM1020>3.0.CO;2-Z

Quantifying tracer levels of ²H₂O enrichment from microliter amounts of plasma and urine by ²H NMR

John G. Jones, Matthew Merritt, Craig Malloy

Research output: Contribution to journal › Article › peer-review

52 Scopus citations

Abstract

A simple and sensitive ²H NMR measurement of ²H₂O enrichment from a 10 μl volume of body fluid is presented. The method allows ²H-enrichment levels of 0.1% or above to be rapidly determined from 10 μl of plasma or urine. The measurement is insensitive to the presence of plasma protein, allowing direct analysis of ²H₂O enrichment from native plasma samples.

Original language	English (US)
Pages (from-to)	156-158
Number of pages	3
Journal	Magnetic resonance in medicine
Volume	45
Issue number	1
DOIs	https://doi.org/10.1002/1522-2594(200101)45:1<156::AID-MRM1020>3.0.CO;2-Z
State	Published - 2001

Keywords

Biosynthetic pathways
Body water
Deuterium
Gluconeogenesis
Tracer

ASJC Scopus subject areas

Radiology Nuclear Medicine and imaging

Access to Document

10.1002/1522-2594(200101)45:1<156::AID-MRM1020>3.0.CO;2-Z

Cite this

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abstract = "A simple and sensitive 2H NMR measurement of 2H2O enrichment from a 10 μl volume of body fluid is presented. The method allows 2H-enrichment levels of 0.1% or above to be rapidly determined from 10 μl of plasma or urine. The measurement is insensitive to the presence of plasma protein, allowing direct analysis of 2H2O enrichment from native plasma samples.",

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