Quantitation of oxypurines and allopurinol metabolites in biological fluids by cation-exchange chromatography

Lawrence Sweetman, William L. Nyhan

Research output: Contribution to journalArticlepeer-review

19 Scopus citations

Abstract

Chromatography of urine, cerebrospinal fluid, and deproteinized serum on 30 × 0.9 cm columns of AG 50 permits the convenient separation of hypoxanthine, xanthine, allopurinol, and its metabolites. Quantitation by determination of absorbance at 250 mμ is accurate and can be automated. Column preparation is simple, and the columns can be regenerated and used daily for several months with no loss of resolution.

Original languageEnglish (US)
Pages (from-to)358-365
Number of pages8
JournalAnalytical biochemistry
Volume31
Issue numberC
DOIs
StatePublished - 1969
Externally publishedYes

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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