TY - JOUR
T1 - Quantitation of oxypurines and allopurinol metabolites in biological fluids by cation-exchange chromatography
AU - Sweetman, Lawrence
AU - Nyhan, William L.
N1 - Funding Information:
‘This investigation was supported by U. S. Public Health Service Research Grant HD 02609 and Training Grant Tl HD 76 from the National Institute of Child Health and Human Development, National Institutes of Health.
PY - 1969
Y1 - 1969
N2 - Chromatography of urine, cerebrospinal fluid, and deproteinized serum on 30 × 0.9 cm columns of AG 50 permits the convenient separation of hypoxanthine, xanthine, allopurinol, and its metabolites. Quantitation by determination of absorbance at 250 mμ is accurate and can be automated. Column preparation is simple, and the columns can be regenerated and used daily for several months with no loss of resolution.
AB - Chromatography of urine, cerebrospinal fluid, and deproteinized serum on 30 × 0.9 cm columns of AG 50 permits the convenient separation of hypoxanthine, xanthine, allopurinol, and its metabolites. Quantitation by determination of absorbance at 250 mμ is accurate and can be automated. Column preparation is simple, and the columns can be regenerated and used daily for several months with no loss of resolution.
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U2 - 10.1016/0003-2697(69)90276-0
DO - 10.1016/0003-2697(69)90276-0
M3 - Article
C2 - 5380707
AN - SCOPUS:0014583678
SN - 0003-2697
VL - 31
SP - 358
EP - 365
JO - Analytical biochemistry
JF - Analytical biochemistry
IS - C
ER -