Quantitation of spinal cord demyelination, remyelination, atrophy, and axonal loss in a model of progressive neurologic injury

Dorian B. McGavern, Paul D. Murray, Moses Rodriguez

Research output: Contribution to journalArticlepeer-review

64 Scopus citations

Abstract

Spinal cord pathology, such as demyelination and axonal loss, is a common feature in multiple models of central nervous system (CNS) injury and disease. Development of methods to quantify spinal cord pathology objectively would aid studies designed to establish mechanisms of damage, correlate pathology with neurologic function, and assess therapeutic interventions. In this study, we describe sensitive methods to objectively quantify spinal cord demyelination, remyelination, atrophy, and axonal loss following the initiation of a progressive inflammatory demyelinating disease with Theiler's murine encephalomyelitis virus (TMEV). Spinal cord demyelination, remyelination, and atrophy were quantified from representative 1-μ-thick cross sections embedded in Araldite plastic using interactive image analysis. In addition, this study demonstrates novel, automated methodology to quantify axonal loss from areas of normal-appearing white matter, as a measure of secondary axonal injury following demyelination. These morphologic methods, which are applicable to various models of CNS injury, provide an innovative way to assess the benefits of therapeutic agents, to determine mechanisms of spinal cord damage, or to establish a correlation with sensitive measures of neurologic function.

Original languageEnglish (US)
Pages (from-to)492-504
Number of pages13
JournalJournal of Neuroscience Research
Volume58
Issue number4
DOIs
StatePublished - Nov 15 1999

Keywords

  • Morphology
  • Multiple sclerosis
  • Myelin diseases
  • Neuropathology
  • Theiler's virus

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience

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