Quantitative measurement of calcium flux through muscle and neuronal nicotinic acetylcholine receptors

Steven Vernino, Marc Rogers, Kristofer A. Radcliffe, John A. Dani

Research output: Contribution to journalArticle

135 Citations (Scopus)

Abstract

A new approach was developed to determine quantitatively the fraction of current carried by Ca2+ through an ion channel under physiological conditions. This approach entails the simultaneous measurement of membrane current and intracellular Ca2+ for single cells. Whole-cell patch-clamp techniques were used to measure current, and intracellular Ca2+ was monitored with the fluorescent indicator fura-2. To obtain a quantitative measure of the fraction of current carried by Ca2+, a cell-by-cell calibration method was devised to account for differences among cells in such factors as cellular volume and Ca2+ buffering. The method was used to evaluate the Ca2+ flux through muscle and neuronal nicotinic ACh receptors (nAChRs). In a solution containing 2.5 mM Ca2+ at a holding potential of - 50 mV, Ca2+ carries 2.0% of the inward current through muscle nAChRs from BC3H1 cells and 4.1% of the inward current through neuronal nAChRs from adrenal chromaffin cells. The Ca2+ flux through neuronal nAChRs of adrenal chromaffin cells is insensitive to α-bungarotoxin. The influx of Ca2+ is voltage dependent, and because of the Ca2+ concentration difference across the cellular membrane, there is Ca2+ influx into the cell even when there is a large net outward current. At both muscle and neuronal cholinergic synapses, activity-dependent Ca2+ influx through nicotinic receptors produces intracellular signals that may have important roles in synaptic development, maintenance, and plasticity.

Original languageEnglish (US)
Pages (from-to)5514-5524
Number of pages11
JournalJournal of Neuroscience
Volume14
Issue number9
StatePublished - Sep 1994

Fingerprint

Nicotinic Receptors
Calcium
Muscles
Cholinergic Receptors
Chromaffin Cells
Bungarotoxins
Intracellular Membranes
Fura-2
Patch-Clamp Techniques
Ion Channels
Synapses
Cholinergic Agents
Calibration
Maintenance
Membranes

Keywords

  • cholinergic
  • fura-2
  • ion channel
  • patch clamp
  • permeability
  • selectivity
  • synaptic transmission

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Quantitative measurement of calcium flux through muscle and neuronal nicotinic acetylcholine receptors. / Vernino, Steven; Rogers, Marc; Radcliffe, Kristofer A.; Dani, John A.

In: Journal of Neuroscience, Vol. 14, No. 9, 09.1994, p. 5514-5524.

Research output: Contribution to journalArticle

Vernino, Steven ; Rogers, Marc ; Radcliffe, Kristofer A. ; Dani, John A. / Quantitative measurement of calcium flux through muscle and neuronal nicotinic acetylcholine receptors. In: Journal of Neuroscience. 1994 ; Vol. 14, No. 9. pp. 5514-5524.
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