Abstract
A limiting dilution assay was used to determine the frequencies of T cells that could be induced to proliferate and kill by Con A and TCGF. The frequency of proliferating cells was 1 in 5 nylon wool-purified spleen cells and 1 in 7 normal lymph node cells. The frequencies in the same cell populations of lectin-dependent killer cell precursors were 1 in 5 and 1 in 9, respectively. Even at limiting numbers of T cells seeded per culture, very few cultures proliferated without showing cytotoxic activity. Thus, under these conditions Con A and TCGF, preferentially if not exclusively, activate T cells that will kill in the presence of PHA. This experimental system can be used for estimating the repertoires of specific CTL-precursors activated by Con A and expanded in TCGF.
Original language | English (US) |
---|---|
Pages (from-to) | 1081-1085 |
Number of pages | 5 |
Journal | Journal of Immunology |
Volume | 127 |
Issue number | 3 |
State | Published - Jan 1 1981 |
ASJC Scopus subject areas
- Immunology and Allergy
- Immunology