Abstract
A limiting dilution assay was used to determine the frequencies of T cells that could be induced to proliferate and kill by Con A and TCGF. The frequency of proliferating cells was 1 in 5 nylon wool-purified spleen cells and 1 in 7 normal lymph node cells. The frequencies in the same cell populations of lectin-dependent killer cell precursors were 1 in 5 and 1 in 9, respectively. Even at limiting numbers of T cells seeded per culture, very few cultures proliferated without showing cytotoxic activity. Thus, under these conditions Con A and TCGF, preferentially if not exclusively, activate T cells that will kill in the presence of PHA. This experimental system can be used for estimating the repertoires of specific CTL-precursors activated by Con A and expanded in TCGF.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1081-1085 |
| Number of pages | 5 |
| Journal | Journal of Immunology |
| Volume | 127 |
| Issue number | 3 |
| State | Published - 1981 |
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ASJC Scopus subject areas
- Immunology
Cite this
Quantitative studies on concanavalin A-induced, TCGF-reactive T cells. I. Correlation between proliferation and lectin-dependent cytolytic activity. / Larsson, E. L.; Lindahl, K. F.; Langhorne, J.; Coutinho, A.
In: Journal of Immunology, Vol. 127, No. 3, 1981, p. 1081-1085.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Quantitative studies on concanavalin A-induced, TCGF-reactive T cells. I. Correlation between proliferation and lectin-dependent cytolytic activity
AU - Larsson, E. L.
AU - Lindahl, K. F.
AU - Langhorne, J.
AU - Coutinho, A.
PY - 1981
Y1 - 1981
N2 - A limiting dilution assay was used to determine the frequencies of T cells that could be induced to proliferate and kill by Con A and TCGF. The frequency of proliferating cells was 1 in 5 nylon wool-purified spleen cells and 1 in 7 normal lymph node cells. The frequencies in the same cell populations of lectin-dependent killer cell precursors were 1 in 5 and 1 in 9, respectively. Even at limiting numbers of T cells seeded per culture, very few cultures proliferated without showing cytotoxic activity. Thus, under these conditions Con A and TCGF, preferentially if not exclusively, activate T cells that will kill in the presence of PHA. This experimental system can be used for estimating the repertoires of specific CTL-precursors activated by Con A and expanded in TCGF.
AB - A limiting dilution assay was used to determine the frequencies of T cells that could be induced to proliferate and kill by Con A and TCGF. The frequency of proliferating cells was 1 in 5 nylon wool-purified spleen cells and 1 in 7 normal lymph node cells. The frequencies in the same cell populations of lectin-dependent killer cell precursors were 1 in 5 and 1 in 9, respectively. Even at limiting numbers of T cells seeded per culture, very few cultures proliferated without showing cytotoxic activity. Thus, under these conditions Con A and TCGF, preferentially if not exclusively, activate T cells that will kill in the presence of PHA. This experimental system can be used for estimating the repertoires of specific CTL-precursors activated by Con A and expanded in TCGF.
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UR - http://www.scopus.com/inward/citedby.url?scp=0019407567&partnerID=8YFLogxK
M3 - Article
VL - 127
SP - 1081
EP - 1085
JO - Journal of Immunology
JF - Journal of Immunology
SN - 0022-1767
IS - 3
ER -