Quantitative studies on concanavalin A-induced, TCGF-reactive T cells. I. Correlation between proliferation and lectin-dependent cytolytic activity

E. L. Larsson, K. F. Lindahl, J. Langhorne, A. Coutinho

Research output: Contribution to journalArticle

39 Scopus citations

Abstract

A limiting dilution assay was used to determine the frequencies of T cells that could be induced to proliferate and kill by Con A and TCGF. The frequency of proliferating cells was 1 in 5 nylon wool-purified spleen cells and 1 in 7 normal lymph node cells. The frequencies in the same cell populations of lectin-dependent killer cell precursors were 1 in 5 and 1 in 9, respectively. Even at limiting numbers of T cells seeded per culture, very few cultures proliferated without showing cytotoxic activity. Thus, under these conditions Con A and TCGF, preferentially if not exclusively, activate T cells that will kill in the presence of PHA. This experimental system can be used for estimating the repertoires of specific CTL-precursors activated by Con A and expanded in TCGF.

Original languageEnglish (US)
Pages (from-to)1081-1085
Number of pages5
JournalJournal of Immunology
Volume127
Issue number3
StatePublished - Jan 1 1981

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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