RAD9-dependent G1 arrest defines a second checkpoint for damaged DNA in the cell cycle of Saccharomyces cerevisiae

Wolfram Siede, Andrew S. Friedberg, Errol C. Friedberg

Research output: Contribution to journalArticle

176 Scopus citations

Abstract

Exposure of the yeast Saccharomyces cerevisiae to ultraviolet (UV) light, the UV-mimetic chemical 4-nitroquinoline-1-oxide (4NQO), or γ radiation after release from G1 arrest induced by α factor results in delayed resumption of the cell cycle. As is the case with G2 arrest following ionizing radiation damage [Weinert, T. A. & Hartwell, L. H. (1988) Science 241, 317-322], the normal execution of DNA damage-induced G1 arrest depends on a functional yeast RAD9 gene. We suggest that the RAD9 gene product may interact with cellular components common to the G1/S and G2/M transition points in the cell cycle of this yeast. These observations define a checkpoint in the eukaryotic cell cycle that may facilitate the repair of lesions that are otherwise processed to lethal and/or mutagenic damage during DNA replication. This checkpoint apparently operates after the mating pheromone-induced G1 arrest point but prior to replicative DNA synthesis, S phase-associated maximal induction of histone H2A mRNA, and bud emergence.

Original languageEnglish (US)
Pages (from-to)7985-7989
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume90
Issue number17
DOIs
StatePublished - Sep 1 1993

Keywords

  • DNA repair
  • UV radiation

ASJC Scopus subject areas

  • General

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