Radioimmunoassay of digitonin

Gary Brooker, John W. Fleming, Elliott M. Ross

Research output: Contribution to journalArticle

Abstract

We describe a radioimmunoassay for digitonin which utilizes the ability of digotonin to compete with 125I-labeled digoxigenin for binding to anti-digoxin antiserum. As performed using the Gammaflo automated radioimmunoassay system and commercially available reagents, the assay can detect as little as 20 μg/ml (15 μM) digitonin. The assay is insensitive to interference by cholesterol or other cell membrane constituents and is useable above and below the critical micelle concentration of digitonin. It should be useful for the monitoring of digitonin concentrations in solubilized biochemical preparations.

Original languageEnglish (US)
Pages (from-to)39-43
Number of pages5
JournalBBA - Biomembranes
Volume685
Issue number1
DOIs
StatePublished - Feb 8 1982

Keywords

  • Digitonin assay
  • Radioimmunoassay

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Cell Biology

Fingerprint Dive into the research topics of 'Radioimmunoassay of digitonin'. Together they form a unique fingerprint.

  • Cite this