Radioimmunoassay of digitonin

Gary Brooker, John W. Fleming, Elliott M. Ross

Research output: Contribution to journalArticle

Abstract

We describe a radioimmunoassay for digitonin which utilizes the ability of digotonin to compete with 125I-labeled digoxigenin for binding to anti-digoxin antiserum. As performed using the Gammaflo automated radioimmunoassay system and commercially available reagents, the assay can detect as little as 20 μg/ml (15 μM) digitonin. The assay is insensitive to interference by cholesterol or other cell membrane constituents and is useable above and below the critical micelle concentration of digitonin. It should be useful for the monitoring of digitonin concentrations in solubilized biochemical preparations.

Original languageEnglish (US)
Pages (from-to)39-43
Number of pages5
JournalBBA - Biomembranes
Volume685
Issue number1
DOIs
StatePublished - Feb 8 1982

Fingerprint

Digitonin
Radioimmunoassay
Assays
Digoxigenin
Critical micelle concentration
Digoxin
Micelles
Cell membranes
Immune Sera
Cholesterol
Cell Membrane
Monitoring

Keywords

  • Digitonin assay
  • Radioimmunoassay

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Medicine(all)

Cite this

Radioimmunoassay of digitonin. / Brooker, Gary; Fleming, John W.; Ross, Elliott M.

In: BBA - Biomembranes, Vol. 685, No. 1, 08.02.1982, p. 39-43.

Research output: Contribution to journalArticle

Brooker, Gary ; Fleming, John W. ; Ross, Elliott M. / Radioimmunoassay of digitonin. In: BBA - Biomembranes. 1982 ; Vol. 685, No. 1. pp. 39-43.
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