Radiolabeled Amino Acid Uptake Assays in Primary Bone Cells and Bone Explants

Leyao Shen, Courtney M. Karner

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Radiolabeled amino acid uptake assays are a highly sensitive method used to characterize the uptake of amino acids by cells or tissues in culture. This method is an excellent tool to quantify changes in amino acid consumption that are associated with states of cellular differentiation and/or disease. The methods presented here can be adapted to measure the transport of all amino acids and can be applied to cultured cells and bone explants.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages449-456
Number of pages8
DOIs
StatePublished - 2021
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume2230
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • H
  • Amino acid uptake
  • Bone explants
  • Primary cells
  • Radiolabeled isotopes
  • Tritium

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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