Ral GTPases Regulate Exocyst Assembly through Dual Subunit Interactions

Serge Moskalenko, Chao Tong, Carine Rosse, Gladys Mirey, Etienne Formstecher, Laurent Daviet, Jacques Camonis, Michael A. White

Research output: Contribution to journalArticle

161 Citations (Scopus)

Abstract

Ral GTPases have been implicated in the regulation of a variety of dynamic cellular processes including proliferation, oncogenic transformation, actin-cytoskeletal dynamics, endocytosis, and exocytosis. Recently the Sec6/8 complex, or exocyst, a multisubunit complex facilitating post-Golgi targeting of distinct subclasses of secretory vesicles, has been identified as a bona fide Ral effector complex. Ral GTPases regulate exocyst-dependent vesicle trafficking and are required for exocyst complex assembly. Sec5, a membrane-associated exocyst subunit, has been identified as a direct target of activated Ral; however, the mechanism by which Ral can modulate exocyst assembly is unknown. Here we report that an additional component of the exocyst, Exo84, is a direct target of activated Ral. We provide evidence that mammalian exocyst components are present as distinct subcomplexes on vesicles and the plasma membrane and that Ral GTPases regulate the assembly interface of a full octameric exocyst complex through interaction with Sec5 and Exo84.

Original languageEnglish (US)
Pages (from-to)51743-51748
Number of pages6
JournalJournal of Biological Chemistry
Volume278
Issue number51
DOIs
StatePublished - Dec 19 2003

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GTP Phosphohydrolases
Exocytosis
Secretory Vesicles
Cell membranes
Endocytosis
Actins
Cell Membrane
Membranes

ASJC Scopus subject areas

  • Biochemistry

Cite this

Moskalenko, S., Tong, C., Rosse, C., Mirey, G., Formstecher, E., Daviet, L., ... White, M. A. (2003). Ral GTPases Regulate Exocyst Assembly through Dual Subunit Interactions. Journal of Biological Chemistry, 278(51), 51743-51748. https://doi.org/10.1074/jbc.M308702200

Ral GTPases Regulate Exocyst Assembly through Dual Subunit Interactions. / Moskalenko, Serge; Tong, Chao; Rosse, Carine; Mirey, Gladys; Formstecher, Etienne; Daviet, Laurent; Camonis, Jacques; White, Michael A.

In: Journal of Biological Chemistry, Vol. 278, No. 51, 19.12.2003, p. 51743-51748.

Research output: Contribution to journalArticle

Moskalenko, S, Tong, C, Rosse, C, Mirey, G, Formstecher, E, Daviet, L, Camonis, J & White, MA 2003, 'Ral GTPases Regulate Exocyst Assembly through Dual Subunit Interactions', Journal of Biological Chemistry, vol. 278, no. 51, pp. 51743-51748. https://doi.org/10.1074/jbc.M308702200
Moskalenko S, Tong C, Rosse C, Mirey G, Formstecher E, Daviet L et al. Ral GTPases Regulate Exocyst Assembly through Dual Subunit Interactions. Journal of Biological Chemistry. 2003 Dec 19;278(51):51743-51748. https://doi.org/10.1074/jbc.M308702200
Moskalenko, Serge ; Tong, Chao ; Rosse, Carine ; Mirey, Gladys ; Formstecher, Etienne ; Daviet, Laurent ; Camonis, Jacques ; White, Michael A. / Ral GTPases Regulate Exocyst Assembly through Dual Subunit Interactions. In: Journal of Biological Chemistry. 2003 ; Vol. 278, No. 51. pp. 51743-51748.
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