RalB mobilizes the exocyst to drive cell migration

Carine Rossé, Anastassia Hatzoglou, Maria Carla Parrini, Michael A. White, Philippe Chavrier, Jacques Camonis

Research output: Contribution to journalArticle

104 Scopus citations

Abstract

The Ras family GTPases RalA and RalB have been defined as central components of the regulatory machinery supporting tumor initiation and progression. Although it is known that Ral proteins mediate oncogenic Ras signaling and physically and functionally interact with vesicle trafficking machinery, their mechanistic contribution to oncogenic transformation is unknown. Here, we have directly evaluated the relative contribution of Ral proteins and Ral effector pathways to cell motility and directional migration. Through loss-of-function analysis, we find that RalA is not limiting for cell migration in normal mammalian epithelial cells. In contrast, RalB and the Sec6/8 complex or exocyst, an immediate downstream Ral effector complex, are required for vectorial cell motility. RalB expression is required for promoting both exocyst assembly and localization to the leading edge of moving cells. We propose that RalB regulation of exocyst function is required for the coordinated delivery of secretory vesicles to the sites of dynamic plasma membrane expansion that specify directional movement.

Original languageEnglish (US)
Pages (from-to)727-734
Number of pages8
JournalMolecular and cellular biology
Volume26
Issue number2
DOIs
StatePublished - Jan 1 2006

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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    Rossé, C., Hatzoglou, A., Parrini, M. C., White, M. A., Chavrier, P., & Camonis, J. (2006). RalB mobilizes the exocyst to drive cell migration. Molecular and cellular biology, 26(2), 727-734. https://doi.org/10.1128/MCB.26.2.727-734.2006