Raman spectroscopy, a potential tool in diagnosis and prognosis of castration-resistant prostate cancer

Lei Wang, Dalin He, Jin Zeng, Zhenfeng Guan, Qiang Dang, Xinyang Wang, Jun Wang, Liqing Huang, Peilong Cao, Guanjun Zhang, Jertong Hsieh, Jinhai Fan

Research output: Contribution to journalArticlepeer-review

27 Scopus citations

Abstract

Purpose: We evaluated the feasibility of Raman spectroscopy (RS) in diagnosis and prognosis of castration-resistant prostate cancer (CRPC) in patients with prostate cancer (PC). Materials and methods: Raman spectra are detected from PC cell lines (LNCaP and C4-2) and tissues using a Labram HR 800 RS. Then, principal component analysis (PCA) and support vector machine (SVM) are applied for prediction. A leave-one-out cross-validation is used to train and test the SVM. Results: There are 50 qualified patients, including 33 with androgendependent prostate cancer (ADPC) and 17 with CRPC. The spectral changes at 1126, 1170, 1315 to 1338, and 1447 cm-1 between CRPC and ADPC are detected in both cells and tissues models, which are assigned to specific amino acids and DNA. PCA/SVM algorithm provided a sensitivity of 88.2% and a specificity of 87.9% for diagnosing CRPC tissues. Furthermore, 14 patients with ADPC progressed to CRPC within 12 months. These patients are separated into two groups depending on whether their cancers progressed to CRPC within 12 months. PCA/SVM could differentiate these two groups with a sensitivity of 85.7% and a specificity of 88.9%. Conclusions: RS has the potential in diagnosis and prognosis of CRPC in clinical practice.

Original languageEnglish (US)
Article number087001
JournalJournal of biomedical optics
Volume18
Issue number8
DOIs
StatePublished - Aug 1 2013

Keywords

  • Raman spectroscopy
  • androgen-dependent prostate cancer
  • castration-resistant prostate cancer
  • prediction

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Biomedical Engineering

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