Rapid assay of lactoferrin in keratoconjunctivitis sicca

C. J. McCollum; G. N. Foulks; B. Bodner; J. Shepard; K. Daniels; V. Gross; L. Kelly; H. D. Cavanagh

doi:10.1097/00003226-199413060-00007

Rapid assay of lactoferrin in keratoconjunctivitis sicca

C. J. McCollum, G. N. Foulks, B. Bodner, J. Shepard, K. Daniels, V. Gross, L. Kelly, H. D. Cavanagh

Research output: Contribution to journal › Article › peer-review

42 Scopus citations

Abstract

We compared measurement of lactoferrin concentration by the LactoCard solid phase ELISA assay with the LactoPlate radial immunodiffusion assay in tears of normal patients and those with keratoconjunctivitis sicca. The LactoCard, a new lactoferrin assay, allows rapid determination of tear lactoferrin concentration in 10-15 min, a notable improvement over the 3 days required of the LactoPlate. There was no statistically significant difference between the accuracy of the two assays in normal patients nor in those patients with a diagnosis of keratoconjunctivitis sicca. Both assays showed a significant decrease in tear lactoferrin concentration in patients with severe keratoconjunctivitis sicca when compared to normal patients. The LactoCard is a rapid and reliable means of measuring tear lactoferrin concentration in a clinical setting.

Original language	English (US)
Pages (from-to)	505-508
Number of pages	4
Journal	Cornea
Volume	13
Issue number	6
DOIs	https://doi.org/10.1097/00003226-199413060-00007
State	Published - 1994

Keywords

Keratoconjunctivitis sicca
Lactoferrin
Tears

ASJC Scopus subject areas

Ophthalmology

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10.1097/00003226-199413060-00007

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title = "Rapid assay of lactoferrin in keratoconjunctivitis sicca",

abstract = "We compared measurement of lactoferrin concentration by the LactoCard solid phase ELISA assay with the LactoPlate radial immunodiffusion assay in tears of normal patients and those with keratoconjunctivitis sicca. The LactoCard, a new lactoferrin assay, allows rapid determination of tear lactoferrin concentration in 10-15 min, a notable improvement over the 3 days required of the LactoPlate. There was no statistically significant difference between the accuracy of the two assays in normal patients nor in those patients with a diagnosis of keratoconjunctivitis sicca. Both assays showed a significant decrease in tear lactoferrin concentration in patients with severe keratoconjunctivitis sicca when compared to normal patients. The LactoCard is a rapid and reliable means of measuring tear lactoferrin concentration in a clinical setting.",

keywords = "Keratoconjunctivitis sicca, Lactoferrin, Tears",

author = "McCollum, {C. J.} and Foulks, {G. N.} and B. Bodner and J. Shepard and K. Daniels and V. Gross and L. Kelly and Cavanagh, {H. D.}",

year = "1994",

doi = "10.1097/00003226-199413060-00007",

language = "English (US)",

volume = "13",

pages = "505--508",

journal = "Cornea",

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T1 - Rapid assay of lactoferrin in keratoconjunctivitis sicca

AU - McCollum, C. J.

AU - Foulks, G. N.

AU - Bodner, B.

AU - Shepard, J.

AU - Daniels, K.

AU - Gross, V.

AU - Kelly, L.

AU - Cavanagh, H. D.

PY - 1994

Y1 - 1994

N2 - We compared measurement of lactoferrin concentration by the LactoCard solid phase ELISA assay with the LactoPlate radial immunodiffusion assay in tears of normal patients and those with keratoconjunctivitis sicca. The LactoCard, a new lactoferrin assay, allows rapid determination of tear lactoferrin concentration in 10-15 min, a notable improvement over the 3 days required of the LactoPlate. There was no statistically significant difference between the accuracy of the two assays in normal patients nor in those patients with a diagnosis of keratoconjunctivitis sicca. Both assays showed a significant decrease in tear lactoferrin concentration in patients with severe keratoconjunctivitis sicca when compared to normal patients. The LactoCard is a rapid and reliable means of measuring tear lactoferrin concentration in a clinical setting.

AB - We compared measurement of lactoferrin concentration by the LactoCard solid phase ELISA assay with the LactoPlate radial immunodiffusion assay in tears of normal patients and those with keratoconjunctivitis sicca. The LactoCard, a new lactoferrin assay, allows rapid determination of tear lactoferrin concentration in 10-15 min, a notable improvement over the 3 days required of the LactoPlate. There was no statistically significant difference between the accuracy of the two assays in normal patients nor in those patients with a diagnosis of keratoconjunctivitis sicca. Both assays showed a significant decrease in tear lactoferrin concentration in patients with severe keratoconjunctivitis sicca when compared to normal patients. The LactoCard is a rapid and reliable means of measuring tear lactoferrin concentration in a clinical setting.

KW - Keratoconjunctivitis sicca

KW - Lactoferrin

KW - Tears

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DO - 10.1097/00003226-199413060-00007

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AN - SCOPUS:0028149303

SN - 0277-3740

VL - 13

SP - 505

EP - 508

JO - Cornea

JF - Cornea

IS - 6

ER -

Rapid assay of lactoferrin in keratoconjunctivitis sicca

Abstract

Keywords

ASJC Scopus subject areas

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