Rapid growth and metabolism of uropathogenic escherichia coli in relation to urine composition

Larry Reitzer, Philippe Zimmern

Research output: Contribution to journalArticle

Abstract

Uropathogenic Escherichia coli (UPEC) strains cause a majority of urinary tract infections (UTIs). Since UPEC strains can become antibiotic resistant, adjunct or alternate therapies are urgently needed. UPEC strains grow extremely rapidly in patients with UTIs. Thus, this review focuses on the relation between urine composition and UPEC growth and metabolism. Compilation of urinary components from two major data sources suggests the presence of sufficient amino acids and carbohydrates as energy sources and abundant phosphorus, sulfur, and nitrogen sources. In a mouse UTI model, mutants lacking enzymes of the tricarboxylic acid cycle, gluconeogenesis, and the nonoxidative branch of the pentose cycle are less competitive than the corresponding parental strains, which is consistent with amino acids as major energy sources. Other evidence suggests that carbohydrates are required energy sources. UPEC strains in urine ex vivo and in vivo express transporters for peptides, amino acids, carbohydrates, and iron and genes associated with nitrogen limitation, amino acid synthesis, nucleotide synthesis, and nucleotide salvage. Mouse models confirm the requirement for many, but not all, of these genes. Laboratory evolution studies suggest that rapid nutrient uptake without metabolic rewiring is sufficient to account for rapid growth. Proteins and pathways required for rapid growth should be considered potential targets for alternate or adjunct therapies.

Original languageEnglish (US)
Article numbere00101-19
JournalClinical Microbiology Reviews
Volume33
Issue number1
DOIs
StatePublished - Jan 2020

Fingerprint

Uropathogenic Escherichia coli
Urine
Urinary Tract Infections
Growth
Carbohydrates
Amino Acids
Nitrogen
Nucleotides
Pentoses
Amino Acid Transport Systems
Citric Acid Cycle
Gluconeogenesis
Information Storage and Retrieval
Sulfur
Phosphorus
Genes
Iron
Anti-Bacterial Agents
Enzymes
Therapeutics

Keywords

  • Carbon metabolism
  • Escherichia coli
  • Iron metabolism
  • Nitrogen metabolism
  • Nucleotide metabolism
  • Urinary tract infection

ASJC Scopus subject areas

  • Epidemiology
  • Immunology and Microbiology(all)
  • Public Health, Environmental and Occupational Health
  • Microbiology (medical)
  • Infectious Diseases

Cite this

Rapid growth and metabolism of uropathogenic escherichia coli in relation to urine composition. / Reitzer, Larry; Zimmern, Philippe.

In: Clinical Microbiology Reviews, Vol. 33, No. 1, e00101-19, 01.2020.

Research output: Contribution to journalArticle

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