Rapid reuse of readily releasable pool vesicles at hippocampal synapses

Jason L. Pyle, Ege T. Kavalali, Erika S. Piedras-Rentería, Richard W. Tsien

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275 Scopus citations


Functional presynaptic vesicles have been subdivided into readily releasable (RRP) and reserve (RP) pools. We studied recycling properties of RRP vesicles through differential retention of FM1-43 and FM2-10 and by varying the time window for FM dye uptake. Both approaches indicated that vesicles residing in the RRP underwent rapid endocytosis (τ ≃ 1 s), whereas newly recruited RP vesicles were recycled slowly (τ ≃ 30 s). With repeated challenges (hypertonic or electrical stimuli), the ability to release neurotransmitter recovered 10-fold more rapidly than restoration of FM2-10 destaining. Finding neurotransmission in the absence of destaining implied that rapidly endocytosed RRP vesicles were capable of reuse, a process distinct from repopulation from the RP. Reuse would greatly expand the functional capabilities of a limited number of vesicles in CHS terminals, particularly during intermittent bursts of activity.

Original languageEnglish (US)
Pages (from-to)221-231
Number of pages11
Issue number1
StatePublished - Jan 1 2000

ASJC Scopus subject areas

  • Neuroscience(all)

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    Pyle, J. L., Kavalali, E. T., Piedras-Rentería, E. S., & Tsien, R. W. (2000). Rapid reuse of readily releasable pool vesicles at hippocampal synapses. Neuron, 28(1), 221-231. https://doi.org/10.1016/S0896-6273(00)00098-2