Rapid reuse of readily releasable pool vesicles at hippocampal synapses

Jason L. Pyle, Ege T. Kavalali, Erika S. Piedras-Rentería, Richard W. Tsien

Research output: Contribution to journalArticle

275 Scopus citations

Abstract

Functional presynaptic vesicles have been subdivided into readily releasable (RRP) and reserve (RP) pools. We studied recycling properties of RRP vesicles through differential retention of FM1-43 and FM2-10 and by varying the time window for FM dye uptake. Both approaches indicated that vesicles residing in the RRP underwent rapid endocytosis (τ ≃ 1 s), whereas newly recruited RP vesicles were recycled slowly (τ ≃ 30 s). With repeated challenges (hypertonic or electrical stimuli), the ability to release neurotransmitter recovered 10-fold more rapidly than restoration of FM2-10 destaining. Finding neurotransmission in the absence of destaining implied that rapidly endocytosed RRP vesicles were capable of reuse, a process distinct from repopulation from the RP. Reuse would greatly expand the functional capabilities of a limited number of vesicles in CHS terminals, particularly during intermittent bursts of activity.

Original languageEnglish (US)
Pages (from-to)221-231
Number of pages11
JournalNeuron
Volume28
Issue number1
DOIs
StatePublished - Jan 1 2000

ASJC Scopus subject areas

  • Neuroscience(all)

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    Pyle, J. L., Kavalali, E. T., Piedras-Rentería, E. S., & Tsien, R. W. (2000). Rapid reuse of readily releasable pool vesicles at hippocampal synapses. Neuron, 28(1), 221-231. https://doi.org/10.1016/S0896-6273(00)00098-2