Rat spermatogenesis in mouse testis

David E. Clouthier, Mary R. Avarbock, Shanna D. Maika, Robert E Hammer, Ralph L. Brinster

Research output: Contribution to journalArticle

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Abstract

Recently, transplantation of mouse donor spermatogonial stem cells from a fertile testis to an infertile recipient mouse testis was described. The donor cells established spermatogenesis in the seminiferous tubules of the host, and normal spermatozoa were produced. In the most successful transplants, the recipient mice were fertile and sired up to 80 per cent of progeny from donor cells. Here we examine the feasibility of transplanting spermatogonial stem cells from other species to the mouse seminiferous tubule to generate spermatogenesis. Marked testis cells from transgenic rats were transplanted to the testes of immunodeficient mice, and in all of 10 recipient mice (in 19 of 20 testes), rat spermatogenesis occurred. Epididymides of eight mice were examined, and the three from mice with the longest transplants (≥ 110 days) contained rat spermatozoa with normal morphology. The generation of rat spermatogenesis in mouse testes suggests that spermatogonial stem cells of many species could be transplanted, and opens the possibility of xenogeneic spermatogenesis for other species.

Original languageEnglish (US)
Pages (from-to)418-421
Number of pages4
JournalNature
Volume381
Issue number6581
DOIs
StatePublished - 1996

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Spermatogenesis
Testis
Seminiferous Tubules
Stem Cells
Tissue Donors
Spermatozoa
Transgenic Rats
Epididymis
Transplantation
Transplants

ASJC Scopus subject areas

  • General

Cite this

Clouthier, D. E., Avarbock, M. R., Maika, S. D., Hammer, R. E., & Brinster, R. L. (1996). Rat spermatogenesis in mouse testis. Nature, 381(6581), 418-421. https://doi.org/10.1038/381418a0

Rat spermatogenesis in mouse testis. / Clouthier, David E.; Avarbock, Mary R.; Maika, Shanna D.; Hammer, Robert E; Brinster, Ralph L.

In: Nature, Vol. 381, No. 6581, 1996, p. 418-421.

Research output: Contribution to journalArticle

Clouthier, DE, Avarbock, MR, Maika, SD, Hammer, RE & Brinster, RL 1996, 'Rat spermatogenesis in mouse testis', Nature, vol. 381, no. 6581, pp. 418-421. https://doi.org/10.1038/381418a0
Clouthier DE, Avarbock MR, Maika SD, Hammer RE, Brinster RL. Rat spermatogenesis in mouse testis. Nature. 1996;381(6581):418-421. https://doi.org/10.1038/381418a0
Clouthier, David E. ; Avarbock, Mary R. ; Maika, Shanna D. ; Hammer, Robert E ; Brinster, Ralph L. / Rat spermatogenesis in mouse testis. In: Nature. 1996 ; Vol. 381, No. 6581. pp. 418-421.
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