RB reversibly inhibits DNA replication via two temporally distinct mechanisms

Steven P. Angus, Christopher N. Mayhew, David A. Solomon, Wesley A. Braden, Michael P. Markey, Yukiko Okuno, M. Cristina Cardoso, David M. Gilbert, Erik S. Knudsen

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

The retinoblastoma (RB) tumor suppressor is a critical negative regulator of cellular proliferation. Repression of E2F-dependent transcription has been implicated as the mechanism through which RB inhibits cell cycle progression. However, recent data have suggested that the direct interaction of RB with replication factors or sites of DNA synthesis may contribute to its ability to inhibit S phase. Here we show that RB does not exert a cis-acting effect on DNA replication. Furthermore, the localization of RB was distinct from replication foci in proliferating cells. While RB activation strongly attenuated the RNA levels of multiple replication factors, their protein expression was not diminished coincident with cell cycle arrest. During the first 24 h of RB activation, components of the prereplication complex, initiation factors, and the clamp loader complex (replication factor C) remained tethered to chromatin. In contrast, the association of PCNA and downstream components of the processive replication machinery was specifically disrupted. This signaling from RB occurred in a manner dependent on E2F-mediated transcriptional repression. Following long-term activation of RB, we observed the attenuation of multiple replication factors, the complete cessation of DNA synthesis, and impaired replicative capacity in vitro. Therefore, functional distinctions exist between the "chronic" RB-mediated arrest state and the "acute" arrest state. Strikingly, attenuation of RB activity reversed both acute and chronic replication blocks. Thus, continued RB action is required for the maintenance of two kinetically and functionally distinct modes of replication inhibition.

Original languageEnglish (US)
Pages (from-to)5404-5420
Number of pages17
JournalMolecular and Cellular Biology
Volume24
Issue number12
DOIs
StatePublished - Jun 2004

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Retinoblastoma
DNA Replication
Replication Protein C
Peptide Initiation Factors
DNA
Proliferating Cell Nuclear Antigen
Cell Cycle Checkpoints
S Phase
Chromatin
Cell Cycle
Maintenance
Cell Proliferation
RNA

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Angus, S. P., Mayhew, C. N., Solomon, D. A., Braden, W. A., Markey, M. P., Okuno, Y., ... Knudsen, E. S. (2004). RB reversibly inhibits DNA replication via two temporally distinct mechanisms. Molecular and Cellular Biology, 24(12), 5404-5420. https://doi.org/10.1128/MCB.24.12.5404-5420.2004

RB reversibly inhibits DNA replication via two temporally distinct mechanisms. / Angus, Steven P.; Mayhew, Christopher N.; Solomon, David A.; Braden, Wesley A.; Markey, Michael P.; Okuno, Yukiko; Cardoso, M. Cristina; Gilbert, David M.; Knudsen, Erik S.

In: Molecular and Cellular Biology, Vol. 24, No. 12, 06.2004, p. 5404-5420.

Research output: Contribution to journalArticle

Angus, SP, Mayhew, CN, Solomon, DA, Braden, WA, Markey, MP, Okuno, Y, Cardoso, MC, Gilbert, DM & Knudsen, ES 2004, 'RB reversibly inhibits DNA replication via two temporally distinct mechanisms', Molecular and Cellular Biology, vol. 24, no. 12, pp. 5404-5420. https://doi.org/10.1128/MCB.24.12.5404-5420.2004
Angus, Steven P. ; Mayhew, Christopher N. ; Solomon, David A. ; Braden, Wesley A. ; Markey, Michael P. ; Okuno, Yukiko ; Cardoso, M. Cristina ; Gilbert, David M. ; Knudsen, Erik S. / RB reversibly inhibits DNA replication via two temporally distinct mechanisms. In: Molecular and Cellular Biology. 2004 ; Vol. 24, No. 12. pp. 5404-5420.
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