Re-evaluation of the model-free analysis of fast internal motion in proteins using NMR relaxation

Kendra King Frederick, Kim A. Sharp, Nicholas Warischalk, A. Joshua Wand

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

NMR spin relaxation retains a central role in the characterization of the fast internal motion of proteins and their complexes. Knowledge of the distribution and amplitude of the motion of amino acid side chains is critical for the interpretation of the dynamical proxy for the residual conformational entropy of proteins, which can potentially significantly contribute to the entropy of protein function. A popular treatment of NMR relaxation phenomena in macromolecules dissolved in liquids is the so-called model-free approach of Lipari and Szabo. The robustness of the mode-free approach has recently been strongly criticized and the remarkable range and structural context of the internal motion of proteins, characterized by such NMR relaxation techniques, attributed to artifacts arising from the model-free treatment, particularly with respect to the symmetry of the underlying motion. We develop an objective quantification of both spatial and temporal asymmetry of motion and re-examine the foundation of the model-free treatment. Concerns regarding the robustness of the model-free approach to asymmetric motion appear to be generally unwarranted. The generalized order parameter is robustly recovered. The sensitivity of the model-free treatment to asymmetric motion is restricted to the effective correlation time, which is by definition a normalized quantity and not a true time constant and therefore of much less interest in this context. With renewed confidence in the model-free approach, we then examine the microscopic distribution of side chain motion in the complex between calcium-saturated calmodulin and the calmodulin-binding domain of the endothelial nitric oxide synthase. Deuterium relaxation is used to characterize the motion of methyl groups in the complex. A remarkable range of Lipari-Szabo model-free generalized order parameters are seen with little correlation with basic structural parameters such as the depth of burial. These results are contrasted with the homologous complex with the neuronal nitric oxide synthase calmodulin-binding domain, which has distinctly different thermodynamic origins for high affinity binding.

Original languageEnglish (US)
Pages (from-to)12095-12103
Number of pages9
JournalJournal of Physical Chemistry B
Volume112
Issue number38
DOIs
StatePublished - Sep 25 2008

Fingerprint

Nuclear magnetic resonance
proteins
Proteins
Calmodulin
nuclear magnetic resonance
evaluation
calmodulin
Nitric oxide
Entropy
nitric oxide
Nitric Oxide Synthase Type I
Nitric Oxide Synthase Type III
Deuterium
entropy
Relaxation Therapy
Burial
Macromolecules
Amino acids
Calcium
Proxy

ASJC Scopus subject areas

  • Surfaces, Coatings and Films
  • Physical and Theoretical Chemistry
  • Materials Chemistry

Cite this

Re-evaluation of the model-free analysis of fast internal motion in proteins using NMR relaxation. / Frederick, Kendra King; Sharp, Kim A.; Warischalk, Nicholas; Wand, A. Joshua.

In: Journal of Physical Chemistry B, Vol. 112, No. 38, 25.09.2008, p. 12095-12103.

Research output: Contribution to journalArticle

Frederick, Kendra King ; Sharp, Kim A. ; Warischalk, Nicholas ; Wand, A. Joshua. / Re-evaluation of the model-free analysis of fast internal motion in proteins using NMR relaxation. In: Journal of Physical Chemistry B. 2008 ; Vol. 112, No. 38. pp. 12095-12103.
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