Real-time luminescence reporting of circadian gene expression in mammals

Research output: Contribution to journalArticle

116 Citations (Scopus)

Abstract

Luminescence reporters have been used successfully in studies of circadian rhythms. Real-time measurements of circadian variations in gene expression were made in living cells, cultured tissues, and whole organisms. Because this technique is relatively easy and continuous noninvasive measurement from tissue cultures allows for a drastic reduction in the number of experimental animals, we believe this method will become a common technique for studying circadian rhythms. Using a multichannel recording apparatus, it may also become a powerful tool for the discovery of new drugs. In the past, measurements were done using hand-made apparatuses or by modifying commercially available equipment. We, along with other investigators, have developed user-friendly equipment for performing circadian rhythms experiments, and these systems are now available commercially. This article describes the use of luminescence reporters in circadian research and provides detailed methods used in these experiments. One of our goals in this article is to reduce experimental variability in different laboratories by proposing standard protocols.

Original languageEnglish (US)
Article number12
Pages (from-to)288-301
Number of pages14
JournalMethods in Enzymology
Volume393
DOIs
StatePublished - 2005

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Mammals
Circadian Rhythm
Luminescence
Gene expression
Gene Expression
Tissue culture
Time measurement
Equipment and Supplies
Animals
Experiments
Cells
Drug Discovery
Tissue
Cultured Cells
Research Personnel
Pharmaceutical Preparations
Research

ASJC Scopus subject areas

  • Biochemistry

Cite this

Real-time luminescence reporting of circadian gene expression in mammals. / Yamazaki, Shin; Takahashi, Joseph S.

In: Methods in Enzymology, Vol. 393, 12, 2005, p. 288-301.

Research output: Contribution to journalArticle

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