Real-time multi-angle projection imaging of biological dynamics

Bo Jui Chang, James D. Manton, Etai Sapoznik, Theresa Pohlkamp, Tamara S. Terrones, Erik S. Welf, Vasanth S. Murali, Philippe Roudot, Kayley Hake, Lachlan Whitehead, Andrew G. York, Kevin M. Dean, Reto Fiolka

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

We introduce a cost-effective and easily implementable scan unit that converts any camera-based microscope with optical sectioning capability into a multi-angle projection imaging system. Projection imaging reduces data overhead and accelerates imaging by a factor of >100, while also allowing users to readily view biological phenomena of interest from multiple perspectives on the fly. By rapidly interrogating the sample from just two perspectives, our method also enables real-time stereoscopic imaging and three-dimensional particle localization. We demonstrate projection imaging with spinning disk confocal, lattice light-sheet, multidirectional illumination light-sheet and oblique plane microscopes on specimens that range from organelles in single cells to the vasculature of a zebrafish embryo. Furthermore, we leverage our projection method to rapidly image cancer cell morphodynamics and calcium signaling in cultured neurons at rates up to 119 Hz as well as to simultaneously image orthogonal views of a beating embryonic zebrafish heart.

Original languageEnglish (US)
Pages (from-to)829-834
Number of pages6
JournalNature methods
Volume18
Issue number7
DOIs
StatePublished - Jul 2021

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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