Recent progress in analysis of intermediary metabolism by ex vivo 13C NMR

Research output: Contribution to journalReview articlepeer-review

Abstract

Advanced imaging technologies, large-scale metabolomics, and the measurement of gene transcripts or enzyme expression all enable investigations of intermediary metabolism in human patients. Complementary information about fluxes in individual metabolic pathways may be obtained by ex vivo 13C NMR of blood or tissue biopsies. Simple molecules such as 13C-labeled glucose are readily administered to patients prior to surgical biopsies, and 13C-labeled glycerol is easily administered orally to outpatients. Here, we review recent progress in practical applications of 13C NMR to study cancer biology, the response to oxidative stress, gluconeogenesis, triglyceride synthesis in patients, as well as new insights into compartmentation of metabolism in the cytosol. The technical aspects of obtaining the sample, preparing material for analysis, and acquiring the spectra are relatively simple. This approach enables convenient, valuable, and quantitative insights into intermediary metabolism in patients.

Original languageEnglish (US)
JournalNMR in biomedicine
DOIs
StateAccepted/In press - 2022

Keywords

  • C
  • cancer
  • glucose
  • glycerol
  • metabolic syndrome
  • NMR
  • stable isotope

ASJC Scopus subject areas

  • Molecular Medicine
  • Radiology Nuclear Medicine and imaging
  • Spectroscopy

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