TY - JOUR
T1 - Receptors with low affinity for neurosteroids and GABA contribute to tonic inhibition of granule cells in epileptic animals
AU - Rajasekaran, Karthik
AU - Joshi, Suchitra
AU - Sun, Chengsan
AU - Mtchedlishvilli, Zakaria
AU - Kapur, Jaideep
N1 - Funding Information:
This study was supported by NIH grants RO1 NS 040337 and RO1 NS RO1 NS044370 .
PY - 2010/11
Y1 - 2010/11
N2 - Neurosteroid sensitivity of GABAA receptor mediated inhibition of the hippocampal dentate granule cells (DGCs) is reduced in animal models of temporal lobe epilepsy. However, the properties and subunit composition of GABAA receptors mediating tonic inhibition in DGCs of epileptic animals have not been described. In the DGCs of epileptic animals, allopregnanolone and L-655708 sensitivity of holding current was diminished and δ subunit was retained in the endoplasmic reticulum and its surface expression was decreased the in the hippocampus. Ro15-4513 and lanthanum had distinct effects on holding current recorded from DGCs of control and epileptic animals suggesting that the pharmacological properties of GABAA receptors maintaining tonic inhibition in DGCs of epileptic animals were similar α4ΒxΓ2 subunits. Furthermore, surface expression of the α4 subunit increased and a larger fraction of the subunit co-immunoprecipitated with theΓ2 subunit in hippocampi of epileptic animals. Together, these studies revealed that functional αα4ΒxΓ and α5ΒxΓ2 receptors were reduced in the hippocampi of epileptic animals and that novel α4bxΓ2 receptors contributed to the maintenance of tonic inhibition. The presence of α4ΒxΓ2 receptors resulted in low GABA affinity and neurosteroid sensitivity of tonic currents in the DGCs of epileptic animals that could potentially increase seizure vulnerability. These receptors may represent a novel therapeutic target for anticonvulsant drugs without sedative actions.
AB - Neurosteroid sensitivity of GABAA receptor mediated inhibition of the hippocampal dentate granule cells (DGCs) is reduced in animal models of temporal lobe epilepsy. However, the properties and subunit composition of GABAA receptors mediating tonic inhibition in DGCs of epileptic animals have not been described. In the DGCs of epileptic animals, allopregnanolone and L-655708 sensitivity of holding current was diminished and δ subunit was retained in the endoplasmic reticulum and its surface expression was decreased the in the hippocampus. Ro15-4513 and lanthanum had distinct effects on holding current recorded from DGCs of control and epileptic animals suggesting that the pharmacological properties of GABAA receptors maintaining tonic inhibition in DGCs of epileptic animals were similar α4ΒxΓ2 subunits. Furthermore, surface expression of the α4 subunit increased and a larger fraction of the subunit co-immunoprecipitated with theΓ2 subunit in hippocampi of epileptic animals. Together, these studies revealed that functional αα4ΒxΓ and α5ΒxΓ2 receptors were reduced in the hippocampi of epileptic animals and that novel α4bxΓ2 receptors contributed to the maintenance of tonic inhibition. The presence of α4ΒxΓ2 receptors resulted in low GABA affinity and neurosteroid sensitivity of tonic currents in the DGCs of epileptic animals that could potentially increase seizure vulnerability. These receptors may represent a novel therapeutic target for anticonvulsant drugs without sedative actions.
KW - Dentate granule cells
KW - GABA-A receptor
KW - Neurosteroids
KW - Temporal lobe epilepsy
KW - Tonic inhibition
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U2 - 10.1016/j.nbd.2010.07.016
DO - 10.1016/j.nbd.2010.07.016
M3 - Article
C2 - 20682339
AN - SCOPUS:77956522595
SN - 0969-9961
VL - 40
SP - 490
EP - 501
JO - Neurobiology of Disease
JF - Neurobiology of Disease
IS - 2
ER -